艾灸足三里、梁门穴对应激性溃疡大鼠胃黏膜细胞凋亡的干预作用
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易受乡, 彭 艳, 常小荣, 彭 娜, 严 洁,
艾灸;足三里穴;梁门穴;应激性溃疡;胃黏膜血流量;内皮素;多巴胺;细胞凋亡,易受乡,彭艳,常小荣,彭娜,严洁,林亚平,易受乡,通讯作
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易受乡, 彭艳, 常小荣, 彭娜, 严洁, 林亚平, 湖南中医药大学针灸推拿学院 湖南省长沙市 410007
易受乡, 1970年湖南医学院医疗系本科毕业, 主要从事针灸治病机制研究.
国家自然科学基金资助项目, No. 30572310
国家973计划中医理论基础研究专项, No. 2005CB523306-9
通讯作者: 易受乡, 410007, 湖南长沙市韶山中路113号, 湖南中医药大学针灸推拿学院. yishouxiang@yahoo.com.cn
电话: 0731-5381161
收稿日期: 2006-06-30 接受日期: 2006-09-01
Effect of moxibustion at Zusanli and Liangmen point on gastric mucosal cell apoptosis in rats with stress ulcer
Shou-Xiang Yi, Yan Peng, Xiao-Rong Chang, Na Peng, Jie Yan, Ya-Ping Lin
Shou-Xiang Yi, Yan Peng, Xiao-Rong Chang, Na Peng, Jie Yan, Ya-Ping Lin,College of Acupuncture and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China
Supported byNational Natural Science Foundation of China, No. 30572310, and the Major State Basic Research Development Program of China (973 Program), No. 2005CB523306-9
Correspondence to:Shou-Xiang Yi, College of Acupuncture and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China. yishouxiang@yahoo.com.cn
Received:2006-06-30 Accepted:2006-09-01
Abstract
AIM: To explore the effects of moxibustion at Zusanli and Liangmen point on gastric mucosal cell apoptosis in rats with stress-induced ulcer, and analyze the relationship between those effects and the plasma content of dopamine (DA), mucosal expression of endothelin (ET).
METHODS: Sixty healthy Sprague Dawley rats were randomly assigned into 4 groups, named goup A, B, C and D, respectively. The animal model of stress ulcer was established by water immersion and restraint stress. The rats in group A, B, and D served as the restraint, model, and non-accupoint controls, while those in group C received moxibustion at Zusanli and Liangmen point. Immunohistochemical method was used to detect the apoptosis index (AI, ×10-6/μm2) in all the groups, and biological signal analyzer was applied to measure gastric mucosal blood flow (GMBF) ......
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