CHENG Haiª²En1,2,3, ZHANG Yi1, ZHANG Cuiª²Li4, PAN Weiª²Wei2, SHI Hua3, HE Liuª²Xing1, YI Faª²Ping1,2,3, SONG Fangª²Zhou1,2,3

    1Department of Biochemistry and Molecular Biology, 2Chorgqing Key Laboratory of Biochemistry and Molecular Pharmacology, 3Key Laboratory of Ministry of Education for Clinical Laboratory Diagnostics, 4Department of Reproductive Medicine, Public Healthy College, Chongqing Medical University, Chongqing 400016, China

    ¡¾Abstract¡¿ AIM: To study the effect on CaSki cells by inhibiting human papillomavirus (HPV)16 E6 gene expression in CaSki cell. METHODS: The vectors expressing siRNA of HPVª²16 E6 gene were constructed and transfected into CaSki cells. The expression of E6 mRNA was detected by RTª²PCR and the expression of P53 protein by Westen blot. CaSki cell cycle was analyzed by flow cytometry. RESULTS: After transfection, the expression of HPVª²16 E6 mRNA was reduced and the expression of P53 was upª²regulated in CaSki cell line. The proliferation of CaSki cells were inhibited and the cells were arrested at G0ª²G1 stage. CONCLUSION: RNAi can inhibite the expression of HPVª²16 E6 gene in CaSki cells, which upª²regulates the expression of tumor suppression protein P53 and results in growth retardation of CaSki cells. ......