ZHAO Rui1, QI Haoª²Wen1, XIE Baiª²Mei1, LI Zhiª²Chao2

    1Department of Respiratory Medicine, Xijing Hospital, 2Department of Pathophysiology, School of Basic Medicine, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To explore the effects of Jiaomu Oil on interleukinª²4,5 in serum and airway hyperresponsiveness (AHR) of asthmatic model in guinea pig and to provide theoretical basis for treatment of asthma using Jiaomu Oil . METHODS: This experiment included 4 groups: normal control group, asthmatic model group, dexamethasone treatment group and Jiaomu Oil treatment group, involving 10 guinea pigs in each group. Asthmatic models in guinea pigs were constructed by immunization of intraperitoneal injection with saline ovalbumin (OVA) and challenge of inspiration with nebulized OVA. A threeª²way cannula was inserted into the lumen of the trachea for mechanical ventilation and the intratracheal pressure (IP) was measured simultaneously for reflecting airway responsiveness. The levels of ILª²4,5 in serum of guinea pigs were determined using enzymeª²linked immunosorbent assay (ELISA). RESULTS: The levels of ILª²4,5 of asthmatic model group[(38.2¡À3.4) and (344.4¡À21.8) ng/L] were significantly higher than those of normal control group[(19.8¡À1.2) and (77.8¡À26.0) ng/L](P<0.01). The levels of ILª²4,5 of Jiaomu Oil[(22.8¡À3.5) and (89.2¡À38.7) ng/L]and dexamethasone groups[(22.0¡À1.8) and (234.6¡À35.1) ng/L]were both significantly lower than those of asthmatic model group(P<0.01). The level of ILª²4 of Jiaomu Oil group didn¬ðt have any significant difference from that of dexamethasone group(P>0.05), While the level of ILª²5 of Jiaomu Oil group was significantly lower than that of dexamethasone group(P<0.01). The airway responsiveness of asthmatic model group [(0.013¡À0.014) g/L] was significantly higher than that of normal control group [(0.168¡À0.186) g/L, P<0.01]. Compared with that of asthmatic model group, the airway responsiveness of Jiaomu Oil [(0.160¡À0.180) g/L] and dexamethasone group [(0.144¡À0.154) g/L] both decreased significantly (P<0.01), but they didn¬ðt have any statistical significance from that of normal control group (P>0.05). The airway responsiveness of Jiaomu Oil group didn¬ðt have any statistical significance from that of dexamethasone group (P>0.05). CONCLUSION: Jiaomu Oil could efficiently decrease the AHR of asthmatic guinea pig. The mechanism may be that Jiaomu Oil could prevent the production of ILª²4,5 in asthmatic guinea pig. ......