DDFA方案对重症急性胰腺炎大鼠心肌细胞凋亡及Bax, Bcl-2表达的影响
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陆正明, 方驰华, 朱明德, 史学深
重症急性胰腺炎;心肌;细胞凋亡;DDFA方案陆正明,方驰华,朱明德,史学深.DDFA方案对重症急性胰腺炎大鼠心肌细胞凋亡及Bax,Bcl-2表达的影响. 世界华人消化杂志2006;14(3
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陆正明, 方驰华, 朱明德, 史学深, 南方医科大学珠江医院肝胆外科 广东省广州市 510282
陆正明, 2003年南方医科大学硕士生, 主要从事肝胆胰外科疾病的研究.
通讯作者: 方驰华, 510282, 广东省广州市工业大道中, 南方医科大学珠江医院普外科. fch58520@sina.com
收稿日期: 2006-07-23 接受日期: 2006-09-28
Effects of DDFA on cardiac myocyte apoptosis and expression of Bax and Bcl-2 in rats with severe acute pancreatitis
Zheng-Ming Lu, Chi-Hua Fang, Ming-De Zhu, Xue-Shen Shi
Zheng-Ming Lu, Chi-Hua Fang, Ming-De Zhu, Xue-Shen Shi, Department of Hepatobiliary Surgery, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China
Correspondence to: Chi-Hua Fang, Department of General Surgery, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province,China. fch58520@sina.com
Received: 2006-07-23 Accepted: 2006-09-28
Abstract
AIM: To explore the effects of DDFA (dexamethasone, dextran, 5-fluorouracil and appotininum) on the cardiac myocyte apoptosis and expression of Bax and Bcl-2 in rats with severe acute pancreatitis (SAP).
METHODS: A total of 45 rats were randomized into 3 groups: pseudo-operation group (n = 15), SAP group (n = 15) and DDFA treatment group (n = 15). SAP model was established by retrograde injection of 50 g/L sodium taurocholate solution into the bilio-pancreatic duct of rats. At the 6th, 12th and 18th hour after the establishment of models, blood cardiac troponin Ⅰ (cTnⅠ, mg/L) and creatine kinase isoenzyme MB (CK-MB, nkat/L) were determined. The myocardiac tissues were collected for light microscopic observation, and myocyte apoptosis was determined by TUNEL method. The expression of Bax and Bcl-2 was detected by SABC immunohistochemical staining.
RESULTS:The blood cTnⅠ(6 h: 2.14 ± 0.09 vs 0.18 ± 0.07; 12 h: 3.30 ± 0.25 vs 0.20 ± 0.08; 18 h: 3.74 ± 0.42 vs 0.19 ± 0.08) and CK-MB (6 h: 7681 ± 252 vs 3389 ± 204; 12 h: 11576 ± 251 vs 2553 ± 205; 18 h: 12972 ± 458 vs 3522 ± 218) level, the myocyte apoptosis index (6 h: 5 ......
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