固相合成；肽类；MSH；芴甲氧羰基_

Fmoc固相合成法合成黑色素细胞刺激素(MSH)的工艺研究

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固相合成；肽类；MSH；芴甲氧羰基,,固相合成；肽类；MSH；芴甲氧羰基,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Fmoc solidphase synthesis of MSH peptide

    ZHU LiGuo, REN FuPing, CHEN WuLing

    College of Life Science, Northwest University, Xian 710069, China

    【Abstract】 AIM: To study the process and influencing factors of synthesizing the melanocyte stimulating hormone(MSH) peptide by Fmoc solidphase synthesis. METHODS: With Fmoc to protect the αamino acid, TBTU/HOBT/DIEA were used as coupling reagents to synthesize the peptide of MSH. The synthesized peptide was purified by the chromatograph and identified by mass spectrograph. RESULTS: The peptide yield was 63%.The synthesized peptide purity reached to 98.67% after being purified by RPHPLC. The molecular mass of the synthesized peptide was 1663.88 ku by MALDIMS and it was consistent with the theoretic molecular mass of 1664.08 ku. CONCLUTION: This method of synthesis is rapid，simple and effective for the peptide MSH, and its fit for the largescale production.

    【Keywords】 solidphase synthesis; peptides; MSH; Fmoc

    【摘要】目的：研究9芴甲氧羰基(Fmoc)固相合成法合成黑色素细胞刺激素(MSH)工艺的步骤及其影响因素. 方法：采用Fmoc保护α氨基 ......

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