反义Snail转录因子抑制肝癌转移
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刘 海, 王志强
原发性肝癌;转录因子;E钙黏蛋白;转染;反义Snail,刘海,王志强,通讯作者:,InhibitoryeffectsofantisenseSnailonin
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刘海, 中建二局二公司职工医院 河南省洛阳市 471001
王志强, 华中科技大学同济医学院附属同济医院肝脏外科中心 湖北省武汉市 430030
通讯作者: 王志强, 430030, 湖北省武汉市解放大道1095号, 华中科技大学同济医学院附属同济医院肝脏外科中心. wangzq0808@sina.com
电话: 027-83663811 传真: 027-83663400
收稿日期: 2006-09-11 接受日期: 2006-10-11
Inhibitory effects of antisense Snail on invasion of hepatoma carcinoma cells
Hai-Liu, Zhi-Qiang Wang
Hai-Liu,Staff Hospital of the Second Company, the Second Construction and Engineering Bureau of China, Luoyang 471001, Henan Province, China
Zhi-Qiang Wang,Department of Hepatic Surgery Center, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
Correspondence to:Zhi-Qiang Wang, Department of Hepatic Surgery Center, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. wangzq0808@sina.com
Received:2006-09-11 Accepted:2006-10-11
Abstract
AIM: To investigate the inhibitory effects of antisense plasmid Snail on the invasion of hepatoma carcinoma cells in vitro.
METHODS: The expression of Snail and E-cadherin mRNA in hepatoma carcinoma cell lines HepG2 were detected by reverse transcription-polymerase chain reaction (RT-PCR). Antisense Snail plasmid was transfected into HepG2 cells, and then antisense group and control group were established. Lipofectin reagent was used to investigate the relationship between E-cadherin and Snail expression, and the inhibitory effects of antisense Snail mRNA were detected by Transwell motility assay and Matrigel invasion assay.
RESULTS: The expression of Snail mRNA was confirmed by RT-PCR, but E-cadherin mRNA expression was absent. The expression of Snail mRNA were 0.887 ± 0.005, 0.665 ± 0.010, 0.348 ± 0.012 at the 0, 24th, 48th h after transfection, and E-cadherin mRNA expression were 0, 0 ......
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