摘要:目的 探讨日本血吸虫疫苗侯选分子—钙离子激活的中性蛋白激酶(Calpain)的保护性免疫和免疫保护机制。 方法 用重组纯化的Calpain抗原免疫BALB/c小鼠，RT-PCR分析小鼠—氧化氮激酶(iNos.enos.nNos)mRNA的表达;ELISA分析免疫小鼠体外培养脾细胞产生的细胞因子IFN-γ和IL-4的动态变化。 结果 重组Calpain抗原免疫小鼠1周后，小鼠iNos mRNA的表达显著增强;培养24h免疫鼠脾细胞上清中IFN-γ浓度显著高于对照组，而IL-4的产生在免役组与对照组之间无显著性差异。 结论 Calpain抗原能诱导IFN-γ的产生，提示Calpain诱导Th1的免疫应答反应来抗日本血吸虫的感染。

    关键词:日本血吸虫;细胞毒;Calpain;BALB/c小鼠

    Kinetics of cytokine IFN-γand expression of IL-4in BALB/c mice immunized by calpain recombinant proteins.

    LIU Man-qian，GAO Shi-tong，HUANG Da-na，et al.

    (Department of Molecular Biology of Shenzhen Municipal Center for Disease Control and Prevention，Shenzhen518020，Guangdong，P.R.China)

    Abstract:Objective To explore the protective immunity and mechanisms of Calpain，a calcium-activated meutral proteinase as a vaccine candidate molecule. Methods Anti-Calpain sera was prepared by immunizing ALB/c mice with recombinant puri-fied Calpain and mRNA expression of iNos，nNos and eNoswere detected by RT-PCR，and the concentration of of IFN-γand IL-4in spleen cell of culture of immunized mice were analyzed by using ELISA. Results High level of IgG antibody specific to the antigen was observed in mice immunized with recombinant calpain.The concentration of IFN-γin the supernatant of mouse spleen cell24hours after culture was significantly higher than that of control ......