【摘要】 目的 用高效液相色谱法测定注射用肌苷的含量。方法 采用Kromasil C18柱(5μm 4.6mm×250mm)，以甲醇-水(10：90)作流动相，在248nm紫外波长检测。结果 肌苷在4.23～63.48μg/ml范围内有良好的线性关系(r=0.9999)。低、中、高3个浓度的平均回收率(n=2)分别为101.4%、101.8%、101.1%，RSD为0.46%。结论 该法简便、快速、准确，可作为测定注射用肌苷中肌苷的含量。

    【关键词】 注射用肌苷；高效液相色谱法；含量测定

    【Abstract】 Objective A high-performance liquid chromatography (HPLC) was established for the determination of inosine contents in injection. Methods Impurity in the inosine injection could be separated on Kromasil C18 colnmn (5μm 4.6mm×250mm) using methanol-water (10:90) as mobile phase and UV detector at 248nm. Results The linear range was 4.23～63.48μg/ml(r=0.9999).Average recovery in the lower,moderate and higher inosine concentration groups was respectively 101.4%,101.8% and 101.1%(n=2) with RSD of 0.46%. Conclusion The method is simple,rapid and accurate.

    【Key words】 inosine injection;HPLC;concentration determination

    肌苷为微生物发酵制得的辅酶类药物 ......