摘要:目的 检测胸腹水、脑脊液(CSF)脱落细胞端粒酶活性，探讨端粒酶对良、恶性肿瘤鉴别诊断的应用价值。 方法 以TRAP-PCR法对胸腹水和脑脊液脱落细胞进行端粒酶活性的检测。 结果 肝硬化、结核性腹膜炎胸腹水及脑积水脑脊液脱落细胞一般不能检出端粒酶活性，而癌性胸腹水或恶性脑膜瘤端粒酶阳性率为86.9%，显著高于细胞学检查的阳性率(69.5%)。细胞学阳性的标本端粒酶活性均为阳性。 结论 端粒酶活性的检测敏感性较高，对体液良、恶性肿瘤有重要的鉴别诊断价值。

    关键词:端粒酶;胸水;腹水;脑脊液;恶性肿瘤

    The diagnostic value of determination of telomerase activity for differentiation of malignant and benign tumors.

    BAI Shu-jing，YANG Wen-fang，GU Hai-ying.

    (Jilin Provincial Clinical Laboratory Center，Jilin130021，Jilin，P.R.China)

    Abstract:Objective To investigate the differential diagnostic value of telomerase activity detection for benign and malignant tu-mor from pleural fluid or ascites or cerebrospinal fluid(CSF). Methods The activity of cast cells'telomerase from pleural fluid or ascites or Cerebrospinal fluid was detected by TRAP-PCR method. Results Commonly cast cells of hepatocirrhosis，tuberculoid peritonitis pleural effusion or ascites or waterhead cerebrospinal fluid did not have telomerase activitis ......