L精氨酸对高血压大鼠主动脉平滑肌细胞凋亡的影响
高血压;主动脉;血管平滑肌细胞;细胞凋亡;一氧化氮;L精氨酸,,高血压;主动脉;血管平滑肌细胞;细胞凋亡;一氧化氮;L精氨酸,1材料与方法,2
摘要:目的 探讨一氧化氮前体L-精氨酸对肾血管性高血压大鼠主动脉平滑肌细胞凋亡的影响。方法 采用两肾一夹(2K1C)型肾血管性高血压大鼠模型。所有大鼠被随机分为4组(每组10只):假手术对照组;高血压对照组(2K1C组);L精氨酸治疗组,于术后第5周开始给予L精氨酸200 mg·kg-1·d-1;LNAME处理组,于术后第5周开始同时给予LNAME 10 mg·kg-1·d-1及L精氨酸200 mg·kg-1·d-1。采用标准尾套法间接检测清醒大鼠血压。给药8周后,处死大鼠,用比色法测定血浆一氧化氮(NO)含量;分离胸主动脉检测胸主动脉平滑肌细胞凋亡(原位末端标记法)。结果 与假手术组大鼠相比,2K1C组大鼠血压明显升高,胸主动脉平滑肌细胞凋亡指数升高,而血浆NO含量明显降低。应用L精氨酸治疗则明显降低了肾动脉狭窄术后高血压大鼠的血压,升高了血浆NO含量,同时也使胸主动脉平滑肌细胞凋亡指数进一步增大。NOS抑制剂LNAME明显抑制了L精氨酸的上述作用。结论 长疗程L精氨酸治疗可刺激高血压血管平滑肌细胞凋亡增加。关键词:高血压;主动脉;血管平滑肌细胞;细胞凋亡;一氧化氮;L精氨酸
The Effects of Larginine on Apoptosis of Aortic Smooth Muscle Cells in Renovascular Hypertensive Rats
YU Liangzhu,HUA Changlin,HUANG Bilan,et al
(Department of Physiology, Xianning College, Xianning Hubei 437100 China)
ABSTRACT:Objective To investigate the effects of Larginine on apoptosis of aortic smooth muscle cells in renovascular hypertensive rats. Methods The renovascular hypertension model was induced in rats by twokidneyoneclip method.All the rats were randomly divided into four groups (n=10 per group):①the shamoperated control group;②the 2K1C renohypertensive control group;③the LArg group, the 2K1C rats were treated with Larginine at a dose of 200mg·kg-1·d-1 from 5th week after renal artery constriction;④the LNAME group, the 2K1C rats were treated with LNAME (10 mg·kg-1·d-1) plus Larginine (200 mg·kg-1·d-1) from 5th week after renal artery constriction. Indirect blood pressure was measured in conscious rats by the standard tailcuff methods. After treatment with both drugs for 8 weeks, the rats were killed,the plasma nitric oxide level was measured by colorimetric method, and the thoracic aorta was removed to determine apoptosis of aortic smooth muscle cell by using terminal deoxynucleotidyl transferasemediated dUTP nick endlabeling method. Results Compared with the shamoperated control group, the untreated 2K1C renohypertensive rats exhibited an increase in blood pressure and apoptotic index of aortic smooth muscle cell, but a decrease in plasma nitric oxide level. Larginine significantly inhibited the increase in blood pressure,but elevated the plasma nitric oxide level and the apoptotic index of aortic smooth muscle cell in 2K1C renovascular hypertensive rats; and these effects of Larginine can be inhibited by LNAME. Conclusion Longterm treatment with Larginine can stimulate an increase in apoptosis of aortic smooth muscle cell in hypertensive rats. ......
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