[摘要]目的 探讨外周血单个核细胞(PBMCs)中乙型肝炎病毒(HBV) X、 P基因整合与原发性肝细胞癌(HCC)的关系。方法 分离HBsAg阳性的40例原发性肝癌病人PBMCs，提取基因组DNA;分别设计HBV X基因、P基因PCR引物，检测HCC病人PBMCs 基因组中HBV的X基因和P基因的整合情况。结果 HCC病人X基因在PBMCs中的整合率为77.5% (31/40)，P基因整合率为15.0%(6/40)，二者差异有显著性( χ2 =31.43，P < 0.001 )。结论 PBMCs 中存在HBV X、 P基因的整合，X基因片段的整合及表达是HCC发生的主要因素。

    [关键词] 癌，肝细胞;肝炎病毒，乙型;基因;病毒整合

    INTEGRATION OF HBV X AND P GENES IN PBMCs IN PATIENTS WITH PRIMARY HEPATOCELLULAR CARCINOMA

    WANG SHU-XIA ，YAN ZHI-YONG，WANG LI-QIU， et al

    (Department of Radiotherapy， Linyi People's Hospital， Linyi 276003， China)

    [ABSTRACT]ObjectiveTo investigate the relationship between primary liver cancer (PLC) and integration of HBV X gene and P gene in peripheral blood mononuclear cells (PBMCs) in PLC patients. MethodsPrimers X1， X2 were designed for HBV X Gene and P1， P2 for P gene. Extracted DNA from isolated PBMCs in 40 PLC patients with HBsAg positive. PCR was used to determine the integration of HBV P and X gene. ResultsThe integration of X gene and P gene was 77.5% (31/40) and 15.0% (6/40) respectively. The difference was significant ( χ2 =31.43 ......