Expression and biological activity of mouse Wntª²3a gene in NIH3T3 cells

    YIN Zongª²Sheng, ZHANG Hui, ZHANG Shengª²Quan, WANG Wei, HUA Xingª²Yi, HU Yong, LI Guangª²Wu

    Department of Orthopaedics, First Affiliated Hospital, Department of Molecular Biology, Institute of Neurobiology, Anhui Medical University, Hefei 230032, China

    ¡¾Abstract¡¿ AIM: To express recombinant mouse Wntª²3a signal protein with biological activity. METHODS: The recombinant eukaryotic expression plasmid, pSecTag2/Hygro Bª²Wnt3a, was transfected into NIH3T3 cells by liposome, then the expressed protein was detected by Western Blot. The saturation density and the capability of suppressing apoptosis in Wnt3a/NIH3T3 cells were evaluated. RESULTS: The Wntª²3a signal protein was stably expressed in Wnt3a/NIH3T3 cells. The saturation density and the capability of suppressing apoptosis in Wnt3a/NIH3T3 cells were markedly increased. CONCLOUSION: We successfully construct the recombinant Wntª²3a signal protein with biological activity.

    ¡¾Keywords¡¿ Wntª²3a; eukaryotic expression; contact inhibition; apoptosis ......