Influence of somatostatin analogue on human hepatocellular cancer cell line MHCC97ª²H

    SUN Hao, SONG Tao, ZHANG Mei, LIU Qingª²Guang, PAN Chengª²En

    First Hospital, Xi¬ðan Jiaotong University, Xi¬ðan 710061,China

    ¡¾Abstract¡¿ AIM: To study the influence of somatostatin analogue octreotide on human hepatocellular cancer cell line MHCC97ª²H and its mechanisms in vivo. METHODS: Twentyª²seven nude mice,of which MHCC97ª²H tumor mass was planted in liver, were divided into 3 groups randomly: negative control group, low dose interfered group, large dose interfered group,and raised at similar condition for 35 d the survival time , the weight at the death point were racorded and the pathologicail changes of livers and lungs were changed; the expression of matrix metalloproteinasesª²2(MMPª²2)in hepatocellular carcinoma was detected by immunohistochemical technique. RESULTS: Compared with the animal models of negative control group, the animal models of interfered groups were heavier in mean weight at death point and lower in metastasis rate (P<0.05); while there was no statistical difference between the 2 interfered groups (P>0.05); the expression of MMPª²2 in hepatocellular carcinoma of interfered groups was gradually increased than that in control group(P<0.05); while there was no statistical difference between interfered groups(P>0.05). CONCLUSION: Octreotide can improve the living quality of animal models and inhibit the metastasis of human hepatocellular cancer cell line MHCC97ª²H in vivo,which might be related to the downª²regulation of the expression of MMPª²2. ......