一种结肠炎相关的结肠癌模型的复制
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李宇华, 刘 莉, 冯 娟, 王庆伟, 孙 阳, 曹 蔚, 王志鹏, 张 蓉,
结肠炎;结肠癌;ICR小鼠;1,2-二甲肼;右旋葡聚糖苷钠,李宇华,刘莉,冯娟,王庆伟,孙阳,曹蔚,王志鹏,张蓉,刘振国,梅其炳,李宇华,通讯作者:,Anovelmodelofcolitis-relatedcolorectalcancer,Yu-HuaL
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李宇华, 刘莉, 冯娟, 王庆伟, 孙阳, 曹蔚, 王志鹏, 张蓉, 刘振国, 梅其炳, 解放军第四军医大学药学系药理教研室 陕西省西安市 710032
李宇华, 硕士研究生, 主要从事分子药理学方面的研究.
通讯作者: 梅其炳, 710032, 陕西省西安市, 解放军第四军医大学药学系药理教研室. qbmei@fmmu.edu.cn
电话: 029-84774552
收稿日期: 2006-10-25 接受日期: 2006-11-28
A novel model of colitis-related colorectal cancer
Yu-Hua Li, Li Liu, Juan Feng, Qing-Wei Wang, Yang Sun, Wei Cao, Zhi-Peng Wang, Rong Zhang, Zhen-Guo Liu, Qi-Bing Mei
Yu-Hua Li, Li Liu, Juan Feng, Qing-Wei Wang, Yang Sun, Wei Cao, Zhi-Peng Wang, Rong Zhang, Zhen-Guo Liu, Qi-Bing Mei,Department of Pharmacology, School of Pharmacy, the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
Correspondence to:Qi-Bing Mei, Department of Pharmacology, School of Pharmacy, the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China. qbmei@fmmu.edu.cn
Received:2006-10-25 Accepted:2006-11-28
Abstract
AIM: To duplicate a colitis-related colorectal cancer model that is cheap, simple and accessible.
METHODS: A total of 320 male ICR mice were randomly divided into 7 experimental groups (group A-G) and 1 control group. Groups A, B and C were given a single intraperitoneal injection of 1, 2-dimethylhydrazine (DMH) at the dose of 10, 15 and 20 mg/kg body weight, respectively. one week after injection, the animals in group A, B and C were treated with 20 g/L dextran sodium sulfate (DSS) in drinking water for consecutive 7 days. Group D, E and F were administered with DMH 10, 15 and 20 mg/kg body weight only, respectively. Group G was given 20 g/L DSS only. The weight and food intake of mice in each group were recorded every week. Several mice were killed to observed the progression to carcinoma of colitis in week 4, 9 and 13. All the mice were executed for observation in week 20.
RESULTS: After administered with DMH and DSS, the bloody stools appeared in 40% mice in week 4, 30% in week 9, and rectal prolapse was observed in 10% mice in week 13, 40% in week 20 ......
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