表皮生长因子介导的核转录因子-κB活化促进胰腺癌细胞尿激酶型纤溶酶原激活物表达及侵袭和转移
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刘 宇, 张 浩, 郭仁宣
表皮生长因子;胰腺癌;尿激酶型纤溶酶原激活物;核转录因子-kB刘宇,张浩,郭仁宣.表皮生长因子介导的核转录因子-kB活化促进胰腺癌细胞尿激酶型纤溶酶原激活物表达及侵袭和转移. 世界华人消化杂志2007;15(4)365-369,表皮生长因子介导的核转录因子-κB活化促进胰腺癌细胞尿激酶型
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刘宇, 张浩, 郭仁宣, 中国医科大学附属第一医院普外二科 辽宁省沈阳市 110001
刘宇, 医学博士, 主要从事肝、胆、胰腺肿瘤的外科治疗.
通讯作者: 张浩, 110001, 辽宁省沈阳市, 中国医科大学附属第一医院普外二科. liuyuhouseman@yahoo.com.cn
电话: 024-83283330
收稿日期: 2006-10-13 接受日期: 2006-11-08
Epidermal growth factor-mediated nuclear factor kappa B activation induces urokinase plasminogen activator expression and invasion, metastasis of pancreatic cancer cells
Yu Liu, Hao Zhang, Ren-Xuan Guo
Yu Liu, Hao Zhang, Ren-Xuan Guo, the Second Department of General Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China
Correspondence to: Hao Zhang, the Second Department of General Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China. liuyuhouseman@yahoo.com.cn
Received: 2006-10-13 Accepted: 2006-11-08
AbstractAIM: To investigate the effect of epidermal growth factor (EGF) on the adhesion, prolifera-tion, and invasion as well as the expression of nuclear factor kappa B (NF-kB) and urokinase plasminogen activator (uPA) of pancreatic cancer cell line KP4.
METHODS: The invasion, adhesion and proliferation of pancreatic cancer cell line KP4 were examined by the corresponding test, respectively. Western blot, reverse transcriptase-polymerase chain reaction (RT-PCR) and electrophoretic mobility shift assay (EMSA) were used to investigate NF-kB activity and uPA expression. The changes of NF-kB activity, uPA expression and invasion of tumor cells were observed as pyrrolidine dithiocarbamate (PDTC), a NF-kB inhibitor, was used.
RESULTS: EGF significantly increased the invasion of KP4 cells in a concentration-dependent way (50, 25, 5 mg/L vs 0 mg/L: 116 ± 13, 97 ± 10, 83 ± 7 vs 72 ± 5; t = 3.552, 3.018, 2.373; P = 0.006, 0.015, 0.042), but did not affect the proliferation or adhesion of the cells ......
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