白藜芦醇对胃癌细胞SGC7901增殖与凋亡的影响
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王学清, 王贺玲, 李 岩, 田 丰, 王 颖
白藜芦醇;胃癌;增值;凋亡;caspase-3王学清,王贺玲,李岩,田丰,王颖.白藜芦醇对胃癌细胞SGC7901增殖与凋亡的影响. 世界华人消化杂志2007;15(4)340-345,王学清,李岩,田丰,王颖,王贺玲,王学清
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王学清, 李岩, 田丰, 王颖, 中国医科大学附属盛京医院消化内科 辽宁省沈阳市 110004
王贺玲, 沈阳市第一人民医院消化内科 辽宁省沈阳市 110044
王学清, 2004年中国医科大学博士在读, 副主任医师, 主要从事胃癌诊治的研究.
通讯作者: 李岩, 110004, 沈阳市和平区三好街36号, 中国医科大学附属盛京医院消化内科. yanli0227@126.com
电话: 024-83956416
收稿日期: 2006-11-03 接受日期: 2006-11-28
Effect of resveratrol on the proliferation and apoptosis of gastric cancer cell line SGC7901
Xue-Qing Wang, He-Ling Wang, Yan Li, Feng Tian, Ying Wang
Xue-Qing Wang, Yan Li, Feng Tian, Ying Wang, Department of Digestive Diseases, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China
He-Ling Wang, Department of Digestive Diseases, the First People’s Hospital of Shenyang City, Shenyang 110044, Liaoning Province, China
Correspondence to: Yan Li, Department of Digestive Diseases, Shengjing Hospital of China Medical University, 36 Sanhao Street, Heping District, Shenyang 110004, Liaoning Province, China. yanli0227@126.com
Received: 2006-11-03 Accepted: 2006-11-28
AbstractAIM:To investigate the effect of resveratrol on the proliferation and apoptosis of gastric cancer cell line SGC7901.
METHODS:Gastric cancer cell line SGC7901 was cultured and treated with different concentrations (0, 10, 20, 50, 100, 200 mmol/L) of resveratrol for 24, 48 and 72 hours. The growth inhibition rate of SGC7901 cells was detected by MTT method, and cell apoptosis was analyzed by flow cytometry (FCM) using annexin V/propidium iodide (PI) double staining. The morphological changes of SGC7901 cells were observed by electron microscopy, and caspase-3 activity was assessed by colorimetric assay.
RESULTS:Resveratrol inhibited the proliferation of SGC7901 cells in a time- and concentration-dependent manner ranging from 20 to 300 mmol/L (P < 0.01). After resveratrol treatment at 0, 10, 20, 50, 100, and 200 mmol/L for 24 hours, the apoptosis rate of SGC7901 cells was 1 ......
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