联合抑制环氧合酶-2与5-脂氧合酶对胃癌细胞增殖凋亡的影响
http://www.100md.com
陈浩莉, 邹来玉, 李建英, 王小众, 陈治新, 黄月红
环氧合酶-2;5-脂氧合酶;联合抑制;AGS细胞;增殖;凋亡陈浩莉,邹来玉,李建英,王小众,陈治新,黄月红.联合抑制环氧合酶-2与5-脂氧合酶对胃癌细胞增殖凋亡的影响. 世界华人消化杂志2007;15(4)323-331,陈浩莉
 |
| 第4页 |
 |
| 第1页 |
参见附件。
陈浩莉, 邹来玉, 李建英, 王小众, 陈治新, 黄月红, 福建医科大学附属协和医院消化内科 福建省福州市 350001
陈浩莉, 福建医科大学附属协和医院2004级硕士研究生, 主要从事消化道肿瘤防治研究.福建省科技人才创新基金资助项目, No. 2002J061
通讯作者: 李建英, 350001, 福建省福州市新权路29号, 福建医科大学附属协和医院消化内科. jyli99@hotmail.com
电话: 0591-83357896-8482
收稿日期: 2006-11-14 接受日期: 2006-11-28
Dual suppression of cyclooxygenase-2 and 5-lipoxygenase inhibits proliferation and induces apoptosis of gastric cancer cell line AGS
Hao-Li Chen, Lai-Yu Zou, Jian-Ying Li, Xiao-Zhong Wang, Zhi-Xin Chen, Yue-Hong Huang
Hao-Li Chen, Lai-Yu Zou, Jian-Ying Li, Xiao-Zhong Wang, Zhi-Xin Chen, Yue-Hong Huang, Department of Gastroenterology, the Affiliated Union Hospital of Fujian Medical University, Fuzhou 350001, Fujian Province, China
Supported by the Innovation Fund for Science and Technology Talent of Fujian Province, No. 2002J061
Correspondence to: Dr. Jian-Ying Li, Department of Gastroenterology, the Affiliated Union Hospital of Fujian Medical University, Fuzhou 350001, China. jyli99@hotmail.com
Received: 2006-11-14 Accepted: 2006-11-28
AbstractAIM:To evaluate the efficacy of the selective cyclooxygenase-2 (COX-2) inhibitor nimesulide, 5-lipoxygenase (5-LOX) inhibitor AA861 and their combination on the proliferation and apoptosis of human gastric carcinoma cell line AGS.
METHODS: Human gastric carcinoma cell line AGS was cultured in RPMI1640 medium with 100 mL/L fetal bovine serum (FBS), 100 kU/L penicillin G and 100 kU/L streptomycin in an incubator at 37°C, with 100% humidity and 50 mL/L CO2. Then AGS cells were treated with AA861 (25, 50, 100 mmol/L) or nimesulide (50, 100, 200 mmol/L) or in combination. The morphological changes of AGS cells were observed and photographed by inverted microscopy. The proliferation of AGS cells was detected by thiazolyl blue tetrazolium bromide (MTT) assays. Acridineorange-ethidiumbromide (AO-EB) fluorescence staining, TUNEL staining and DNA-fragment assay were used to evaluate the apoptotic of AGS cells ......
您现在查看是摘要介绍页，详见PDF附件。