Effect of VHL mRNA and FIHª²1 mRNA expressions in colorectal carcinoma on HIFª²1¦Á protein level

    YANG Xiaoª²Jun1, GAO Peng2, CHE Tuanª²Jie1, HUANG Haiª²Yun2

    1Clinical Medical College, Lanzhou University, 2Department of General Surgery, People¬ðs Hospital of Gansu Province, Lanzhou 730000, China

    ¡¾Abstract¡¿ AIM: To detect mRNA expressions of VHL and FIHª²1 in colorectal cancer, and explore their effect on HIFª²1¦Á protein level. METHODS: Semiquantitative RTª²PCR assay was used to detect the expressions of VHL mRNA, FIHª²1 mRNA in 42 patients with colorectal cancer; Expression of HIFª²1¦Á protein was measured by SP immunohistochemistry; Spearman rank correlation was used to analyze the correlation between them. RESULTS: mRNA levels of VHL and FIHª²1 in colorectal cancer were significantly lower than those in adjacent tissue (t=-10.14, P=0.00£»t=-15.26, P=0.00)£» Both expressions decreased as Dukes stage increased, and the expressions in Dukes A+B stage were significantly higher than those in Dukes C+D stage (t=2.84, P=0.01£» t=-5.13, P=0.00)£» Their expressions had no relationship with tumor location and gender. In 42 cases of colorectal cancer, positive rate of HIFª²1¦Á protein was 69.1% (29/42), the expression in Dukes C+D stage (80.0%, 24/30) was significantly higher than that in Dukes A+B stage (41.7%,5/12), (t=5.89,P=0.02); it was negative in adjacent tissue; Spearman rank correlation displayed that the expressions of VHL mRNA and FIHª²1 mRNA were negatively correlated with the HIFª²1¦Á protein level (rs=-0.97, P=0.01; rs=-0.66, P=0.01). CONCLUSION: The overexpression of HIFª²1¦Á may play an important role in the development and matastasis of colorectal cancer£» The decrease of VHL mRNA and FIHª²1 mRNA expressions is closely correlated with the increase of HIFª²1¦Á protein level in colorectal cancer. ......