Stability test and SPF chick immune test of recombinant adenovirus containing major outer membrane protein of Chlamydia psittaci of chicken origin

    ZHOU Jiª²Zhang, QIU Changª²Qing, ZHANG Xiaoª²Ying, LIN Guoª²Zhen, CAO Xiaoª²An, ZHANG Lin, CHENG Shuª²Min

    Key laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China, Animal Quarantine Station of Lanzhou City, Lanzhou 730050, China

    ¡¾Abstract¡¿ AIM: To detect the stability of the recombinant adenovirus plasmid containing major outer membrane protein (MOMP) gene from Chlamydia psittaci in HEK293 cells, to evaluate whether cross reactivity exists or not between recombinant adenovirus and positive serum of Egg Drop Symdrome (EDS), and to evaluate whether SPF chicks vaccinated with the recombinant adenovirus were protected or not when challenged with virulent CpL strain. METHODS: The recombinant adenovirus plasmids were passaged in HEK293 cells in series and the target gene was detected by PCR. The recombinant adenovirus was mixed with positive serum of EDS in vitro and then infected HEK293 cells. The SPF chicks were vaccinated with recombinant adenovirus and then challenged with the CpL strain. RESULTS: The target gene of recombinant adenovirus plasmid could be amplified by PCR in different passages of HEK293 cells. There was no cross reactivity between recombinant adenovirus and positive serum of EDS. 90% of the SPF chicks vaccinated with recombinant adenovirus were protected when challenged with the CpL strain. CONCLUSION: The recombinant adenovirus plasmid can be passaged stably in HEK293 cells. The recombinant adenovirus can not react with positive serum of EDS. The SPF chicks inoculated with the recombinant adenovirus vaccination are protected. ......