Establishment of acute rejection model of orthotopic liver transplantation in inbred rats

    LIU Jiangª²Wen, TANG Yong, LI Youª²Ping, ZHAO Na, LI Qing, CHENG Nanª²Sheng, YAN L¨¹ª²Nan

    Department of General Surgery, Shihezi People¬ðs Hospital, Shihezi 832000, China, Transplantation Immunity Laboratory, Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China

    ¡¾Abstract¡¿ AIM: To establish the orthotopic liver transplantation models in inbred rats and judge the grades of acute rejection. METHODS: Surgical skills were trained by using closed colony SD and Wistar rats, followed by DA to LEW orthotopic liver transplantation with Kamada¬ðs twoª²cuff technique. The grades of acute rejection were judged by their clinical manifestations and Banff schema. RESULTS: Fifteen models of DA to LEW liver transplantation were established, the success rate being 86.7%. The causes of death were hemorrhage and thrombosis of suprahepatic and infrahepatic vena cava and obstruction of biliary tract. ¢ñgrade rejection occurred at 3rd day and reached the peak at 7th day after transplantation. Remaining models died of ¢ó grade rejection besides of postoperative complication within 12 d after transplantation. CONCLUSION: The acute rejection of DA to LEW orthotopic liver transplantation model is stable and intensive. It can be effectively used to study allograft rejection and tolerance. But it is difficult to establish the kind of orthotopic liver transplantation models in inbred rats because of their own tissue characteristics. ......