肺气肿胶囊；_高效液相色谱法；_槲皮素；_山柰素_

肺气肿胶囊中的槲皮素和山柰素的含量测定

http://www.100md.com 《时珍国医国药》 2006年第10期

肺气肿胶囊；,高效液相色谱法；,槲皮素；,山柰素,,肺气肿胶囊；,高效液相色谱法；,槲皮素；,山柰素,1仪器与材料,2方法与结果,3小结,参考文献：

     摘要：目的建立肺气肿胶囊中槲皮素和山柰素含量测定方法。方法采用高效液相色谱法，使用C18柱，流动相为甲醇-0.4%磷酸溶液(60∶40)，检测波长为370 nm，流速为0.6 ml/min。结果槲皮素和山柰素分别在0.008~0.06 μg和0.006 88~0.0516 μg范围内呈良好线性关系，平均回收率分别为97.34%和97.72%，RSD分别为2.4%和1.3%(n=5)结论该方法灵敏准确，重现性好，专属性强，可作为肺气肿胶囊的质量控制。

    关键词：肺气肿胶囊；高效液相色谱法；槲皮素；山柰素

    Determination of Quercetin and Kaempferol in Feiqizhong Capsules

    YUAN Bin , OUYANG Zhen, MIAO Yadong, JIANG Taotao, SHEN Jiangying

    (College of Biological and Engineering of Jiangsu University, Zhenjiang, Jiangsu 212013, China)

    Abstract：ObjectiveTo establish a method to determine the content of quercetin and kaempferol in Feiqizhong Capsules.MethodsHPLC was used to determine the content of quercetin and kaempferol with detection wavelength at 370nm. The mobile phase consists of a mixture of methanol-0.4%H3PO4(60∶40). The flow rate was 0.6ml/min. ResultsThe linear regression for quercetin and kaempferol was obtained at the range of 0.008~0.006μg and 0.006 88~0.051 6 μg. The average recovery of quercetin and kaempferol were 97.34% and 97.72%, RSD were 1.3% and 1.7% respectively(n=5). ConclusionThe method is sensitive, accurate, reproducible and exclusive. It can be used for quality control of Feiqizhong Capsules. ......

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