摘要：目的制定三棵针药材的鉴别及含量测定方法。方法采用薄层色谱法定性鉴别；高效液相色谱法测定三棵针中盐酸小檗碱的含量。高效液相色谱条件为Vp-ODSC18柱(4.6 mm×150 mm，5 μm)，流动相为乙腈：0.02 mol/L磷酸二氢钾(24：76)；流速为1.0 ml/min；检测波长346 nm；柱温：30℃。 结果薄层色谱鉴别斑点清晰，易于识别；高效液相色谱测定盐酸小檗碱在2.04~20.4 μg/ml范围内呈良好线性关系，平均回收率99.30%，RSD=0.53%。结论该方法简便易行，重线性好，可用于对三棵针药材的质量控制。

    关键词：三棵针； 盐酸小檗碱； 薄层色谱； 高效液相色谱法

    Studies of Quality Control Method on Radix Berberidis

    LIU Peng-han , WEI Geng-long ,LU Lai-xiang ,ZHAO Dian-feng , PAN Sheng-qian

    (Guangxi Tiantianle Pharmaceutical Co. Ltd, Liuzhou 545100,China)

    Abstract：ObjectiveTo study the methods of identification and assay in Radix Berberidis. MethodsUsing TLC to identify and HPLC to determine the content of berberine hydrochloride in Radix Berberidis. The HPLC system consisted of Vp- ODSC18 column (4.6 mm×150 mm,5 μm) ,acetonitrile:0.02 mol/L KH2PO4 (24∶76) mixture as mobile phase, detection wavelength at 346 nm,flow rate of 1.0 ml/min, column temperature：30℃. ResultsThe spots in the TLC were clear and distinguishable, the linear range of berberine hydrochloride was from 2.04 to 20.4 μg/ml.The average recovery was 99.30%, RSD was 0.53%. ConclusionThe methods are simple and accurate, can be used for the quality control of Radix Berberidis. ......