复方丹参注射液对受损伤的血管内皮细胞形态结构的影响
复方丹参注射液；,血管内皮；,扫描电镜,,复方丹参注射液；,血管内皮；,扫描电镜,1材料与方法,2结果,3讨论,参考文献：
摘要：目的探讨复方丹参注射液对氧化所致损伤的血管内皮细胞的保护作用。方法采用脐静脉血管内皮细胞(human umbilical vein cells)，用倒置相差显微镜和扫描电镜观察各组内皮细胞(endothelial cell, EC)的形态学特点。分光光度计测定细胞上清液中丙二醛的含量。结果扫描电镜观察显示过氧化氢组EC皱缩变小，细胞表面干裂，表面微绒毛减少甚至消失，细胞间隙增宽，而经丹参预处理过的过氧化氢组细胞形态结构则明显改善。MDA含量测定表明过氧化氢组的MDA含量明显增高。相反，正常组和丹参组中MDA含量减少(P<0.01)。结论 复方丹参注射液通过其抗氧化作用，对氧化所引起的受损血管EC有保护作用。关键词：复方丹参注射液； 血管内皮； 扫描电镜
Effects of the Compound Injection of Danshen on the Morphological Structure of Injured Vascular Endothelial Cells
REN Xiangshan， SONG Jingyu， LI Zhuhu ， LIN Zhenhua* ， JIN Xianguo
(Department of Pathology, School of Basic Medical Sciences, Yanbian University, Yanji， Jilin 133000, China.Yanbian Hospital of TCM, Yanji, Jilin 133000 China)
Abstract：ObjectiveTo study the protective effects of the compound injection of Dan-shen on cultured endothelial cells (EC) injured by oxidization. MethodsThe morphological characteristics of EC in primary cutured human umbilical vein cells (HUVEC) in vitro were ovserved by the inverted microscope and scanning electron microscopy(SEM). The content of MDA in the supernatant fluid of cultured EC cells was detected by spectrophotometry.ResultsIt was observed by SEM that ECs in the H2O2 group were wrinkle and small, cell membrane had split and aperture, the microvillus decreased or disappeared, stiff or broken, and the cell gaps were wide. However, ECs in the Danshen group was similar with the normal group. MDA content obviously increased in the H2O2 group. However, the content of normal and Danshen group decreased. (P<0.01) .ConclusionThe compound injection of Danshen has protective effects on the injured vascular endothelial cells induced by oxidization through antioxidization function, ......
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