四棱豆；_总黄酮；_提取工艺；_含量____

四棱豆总黄酮含量的研究

http://www.100md.com 《时珍国医国药》 2006年第10期

四棱豆；,总黄酮；,提取工艺；,含量,,四棱豆；,总黄酮；,提取工艺；,含量,1材料与方法,2结果与分析,3讨论,参考文献：

     摘要：目的研究了四棱豆总黄酮的提取工艺和含量。方法采用正交实验分析和分光光度法。结果四棱豆总黄酮的最佳提取工艺是水浴温度为85℃，提取时间为6 h，提取剂乙醇浓度为80%，并且该提取方法稳定性好，精密度和回收率高；以芦丁为对照品，测得四棱豆根、茎、叶、花、果实、种子和整株的总黄酮含量分别为0.73%，0.93%，2.30%，3.96%，2.88%，3.12%和2.44%。结论该研究为四棱豆的综合开发利用提供了科学依据，具有重要的理论和实践指导意义。

    关键词：四棱豆；总黄酮；提取工艺；含量

    Study on the Flavonoid Content in Winged Bean

    QUAN Miaohua ， CHEN Dongming

    (Huaihua Biological College, Huaihua Hunan, 418008，China)

    Abstract:Objective To study the extraction technology and the content of the flavonoid in winged bean. MethodsThe flavonoid content in winged bean was studied by ultravioletvisible spectrophotometry and the orthogonal experiments. ResultsThe analytical result showed that the optimum extracting conditions were: extracting temperature 85℃,extracting time 6h,extraction reagent 80% alcohol.And the stability of the method was very good,meanwhile the precision and the rate of recovery were high. With rutin as standard material,we drew a conclusion that the total contents of flavonoids in the tuberous roots ,stems,leaves,flowers , tender pods,seeds and the whole plant of winged bean were 0.73%,0.93%,2.30%,3.96%,2.88%,3.12% and 2.44% ,respectively.ConclusionThe study provides a scientific basis for the development of winged bean,and has important guiding significance in both theory and practice. ......

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