摘要：目的建立胶束电动毛细管电泳色谱法(MECC)测定半夏及其同科其它品种药材中苯甲酸的含量的方法。方法用MECC法对样品进行分析。毛细管柱内径75 μm，长62.5 cm；运行电压25 kV，检测波长200 nm，分离温度35℃；緩冲液为30 mmol·L��1，SDS��30 mmol·L��1硼酸��15%甲醇(pH值9.5)；压力进样，进样时间3 s。结果苯甲酸在0.044～2.2 mg/ml浓度范围内具有良好的线形关系( r＝0.9994)， 平均回收率为96.7% (RSD=2.4%, n=6), 对半夏及其同科属其它品种共14个样品中的苯甲酸含量进行了测定。结论该方法快速、简便、并且较为准确，可供评价半夏等品种质量时参考。

    关键词：半夏； 掌叶半夏； 水半夏； 苯甲酸 ； 胶束电动毛细管色谱法

    Determination of Benzoic Acid Contents in Rhizoma Pinelliae and Other Species from Araceae by Micellar Eletrokinetic Capillary Chromatography(MECC)

    CAO Yan，HUANG Bi�瞫heng， WANG Ping， CHEN Ke�瞝i*

    (Hubei College of Traditional Chinese Medicine ，Hubei 430061, China)

    Abstract：ObjectiveThe method of micellar electrokinetic capillary chromatography(MECC)was established to determine the contents of benzoic acid in Rhizoma Pinelliae and several Rhizomes from other Araceae species; MethodsThe samples from rhizomes of P. ternana and other species of Araceae were analyzed by MECC; The Capillary column was 62. 5 cm long and 75 μm in internal diameter; The operation voltage 25 kV ; test wavelength 200 nm; column temperature 35 ℃; the mobile buffer was 30 mmol·L��1 SDS��30mmol·L��1 boric acid ��15% methyl alcohol (pH 9. 5); The sampling was conducted by pressure, the sampling time was 3 seconds. ResultsThe linear range of determination for benzoic acid was 0. 044~2.2 mg/ml (r =0. 999 4). The average recovery rate was 96.7%(cv =2.4%,n =6). The contents of benzoic acid in the 14 samples from P. ternana and other species of Araceae were determined. ConclusionThe method is fast, simple, convenient, comparatively accurate, and can be used to refer when appraising quality of Rhizoma Pinelliae and several species. ......