复方厚朴颗粒；_高效液相色谱法；_厚朴酚；_和厚朴酚

高效液相色谱法测定复方厚朴颗粒中厚朴酚及和厚朴酚的含量

http://www.100md.com 《时珍国医国药》 2006年第10期

复方厚朴颗粒；,高效液相色谱法；,厚朴酚；,和厚朴酚,,复方厚朴颗粒；,高效液相色谱法；,厚朴酚；,和厚朴酚,1仪器与试剂,2方法与结果,3讨论,参考文献：

     摘要：目的建立HPLC法测定复方厚朴颗粒中厚朴酚及和厚朴酚的含量。方法采用DiamondsilCMC18色谱柱(6.4 mm×200 mm,5 μm)；流动相为甲醇-0.1%磷酸溶液(78∶22)；流速1 ml/min；柱温35℃；检测波长294 nm。结果厚朴酚的线性范围为0.041 1~0.822 2 μg，r=0.999 9，平均回收率为97.1%,RSD=1.27%；和厚朴酚的线性范围为0.023 06~0.461 2 μg ,r=1，平均回收率为96.7%，RSD=1.25%。结论该方法结果准确，重现性好，可作为复方厚朴颗粒质量控制的定量方法。

    关键词：复方厚朴颗粒；高效液相色谱法；厚朴酚；和厚朴酚

    Determination of Magnolol and Honokiol in Compound Houpu Granule By HPLC

    XIA Houlin ，SHENG Yan，ZHOU Xiaomei，WU Xi，DONG Min，TAN Ling

    (Chengdu University of Traditional Chinese Medicine, 610072 ,China)

    Abstract：ObjectiveTo establish an HPLC method for the determination of magnolol and honokiol in Compound Houpu Granule.MethodsThe samples were analyzed on a DiamondsilCMC18 column (6.4 mm×200 mm,5μm). The mobile phase was a mixture of MeOH-0.1%H3PO4(78:22);the rate of flow was 1ml/min; the column temperature was 35 ℃; the detection wavelength was 294 nm.ResultsCalibration graph was linear in the range of 0.041 1~0.822 2 μg,r=0.999 9 for magnolol, 0.023 06~0.456 12μg, r=1 for honokiol. The recovery was 97.1%, RSD=1.27% for magnolol; 96.7%,RSD=1.25% for honokiol.ConclusionThe method is sensitive and its results are accurate with good reproducibility .It can be used for the quality control of Compound Houpu Granule. ......

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