Effect of allograft kidney tissue mass implantation on EPO of rats with chronic renal failure

    Liu Rongyao1, Wang Dongwen2

    (1. Department of Urology, the People¬ðs Hospital of L¨¹liang, L¨¹liang 03300; 2. Department of Urology, First Hospital of Shanxi Medical University, Taiyuan 030001, China)

    ABSTRACT: Objective To study the effect of kidney tissue mass implantation into the capsule of rat kidney on EPO of rats with chronic renal failure. Methods After the models of chronic renal failue (CRF) induced by adriamyucin were set up in Wistar rats, the kidney tissue mass (1mm3) of newborn Wistar rats were located under the capsule of kidney of the models. During the process of treatment, EPO level in the serum was tested by EPO ELISA kit. Renal pathological changes were observed, and the expression of EPO in transplantation tissue was examined by Immunohistochemical method. Results ¢Ù The levels of EPO (1.160¡À0.324mU/mL) in the control group were lower than those of the other groups, with the difference being significant (P<0.05). ¢Ú The grafts survived and increased in size from 1mm3 to 4mm3 in 60 days, around which there were plenty of newborn blood vessels. Histological examination showed that the grafts had normal structure of glomerule and uriniferous tubules. ¢Û Immunohistochemical study demonstrated EPO positive grains were located in the region of uriniferous tubules and there were more positive grains in renal tissue of the graft than in that of CRF (P<0.05). Conclusion Allograft transplantation of kidney tissue mass can ameliorate renal anemia caused by chronic renal failure and might be a new therapeutic means to treat CRFª²induced anemia. ......