补骨脂药材；_总呋喃香豆素；_紫外分光光度法_

紫外分光光度法测定补骨脂药材中总呋喃香豆素含量2

http://www.100md.com 《时珍国医国药》 2006年第8期

补骨脂药材；,总呋喃香豆素；,紫外分光光度法,,补骨脂药材；,总呋喃香豆素；,紫外分光光度法,1仪器与试药,2方法与结果,3讨论,参考文献：

     摘要：目的建立紫外分光光度法测定补骨脂药材中总呋喃香豆素的含量。方法补骨脂药材粉末经索氏提取2 h，过聚酰胺柱后，采用紫外分光光度法，检测波长为245 nm对补骨脂药材中的总呋喃香豆素进行测定。结果采用该方法可准确测定补骨脂药材中总呋喃香豆素的含量，补骨脂素在1.008～10.08μl/ml范围内吸光度与浓度的线性关系良好，样品平均回收率为98.7%，精密度RSD为0.11%，重复性RSD为2.8%，稳定性RSD为0.75%(4 h内)。结论该方法简单、准确，可用于补骨脂药材中总呋喃香豆素的含量测定。

    关键词：补骨脂药材；总呋喃香豆素；紫外分光光度法

    Determination of the Total Furanocoumarins in Fructus Psoraleae by UV Spectrophotometry

    SHOU Qingyao， YANG Rongping*， WANG Binhao， ZHONG Guoyue， TU Yongqin， HU Yijuan

    (Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China;Chongqing Academy of Chinese Materia Medica,Chongqing 400065,China)

    Abstract：ObjectiveTo establish a UV spectrophotometry for determination of the total furanocoumarins in Fructus Prosraleae. MethodsThe powder of Fructus Psoraleae was extracted by CH3OH for two hours and puerariaed by polyamide column chromatography ;when it was determined by UV spectrophotometry, wave length was 245 nm.ResultsThe method had good linear relationship from 1.008μl/ml to 10.08 μl/ml,and the recovery rate was 98.7%.The relative standard deviation of accurate test and stability test were 2.8%and 0.75% respectively.ConclusionThe method is simple and accurate, and can be used for determination of the total furanocoumarins in Fructus Psoraleae. ......

百拇医药网 http://www.100md.com/html/DirDu/2007/02/17/37/65/59.htm

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