舟山眼镜蛇毒蕈碱样多肽的分离及其对M胆碱受体的激动效应
眼镜蛇毒液类;,受体,胆碱能;,毒蕈碱;,胆碱能激动剂;,放射受体分析法,,眼镜蛇毒液类;,受体,胆碱能;,毒蕈碱;,胆碱能激动剂;,放射受体分析法,1材料与方法,2结果,3讨论,参考文献:
摘要: 目的 从舟山眼镜蛇Naja atra蛇毒中分离毒蕈碱样多肽,探讨其与M胆碱受体的关系。方法 应用分子筛凝胶Sephadex G50、Sephadex G100、离子交换凝胶CMSepharose F.F.分离纯化目的组分;SDSPAGE鉴定目的蛋白的纯度并测定其相对分子质量;放射受体分析法测定该物质与大鼠脑组织M胆碱受体的亲和力;选用离体豚鼠回肠纵行肌观察其对M胆碱受体的效应。结果 从舟山眼镜蛇蛇毒中获得一种与大鼠脑组织M胆碱受体具有高亲和力的多肽(Ki为10.12 nmol/L),其相对分子质量约14 kD。该多肽可引起离体豚鼠回肠纵行肌收缩,此效应可被阿托品阻断。结论 舟山眼镜蛇蛇毒中获得与M胆碱受体具有高亲和力的多肽,该多肽对M胆碱受体具有激动效应。关键词: 眼镜蛇毒液类; 受体,胆碱能; 毒蕈碱; 胆碱能激动剂; 放射受体分析法
The Agonistic Effect of Muscarinic Peptide Isolated from
Naja atra Venom to Muscarinic Cholinergic Receptor
Zheng Jiabing, Huang Lifeng, Xu Ying, Yu Changxi
Department of Pharmacology, College of Pharmacy, Fujian Medical University, Fuzhou 350004, China
ABSTRACT: Objective To isolate muscarinic peptide(MP) from Naja atra venom and to study the effect of MP in muscarinic cholinergic receptor(mChR). Methods MP had been isolated and purified from Naja atra venom by gel filtration on Sephadex G50 and Sephadex G100, chromatography on ionexchanger CMSepharose FF. Homogenicity and molecular weight of the MP was determined on SDSPAGE. Isolated ileal longitudinal muscles of the guinea pigs were used to observe the effect of MP on mChR.The affinity of MP to rat brain synaptosomal membrane was tested by radio receptor assay. Results Muscarinic peptide isolated from Naja atra venom could displace \[3H\]QNB from rat brain. Its molecular weight was 14 kD. In guineapig ileum, MP produced a potent contraction which can be blocked by atropine. Conclusion The muscarinic peptide from Naja atra venom was muscarinic agonist. ......
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