[摘要] 目的: 研究腺病毒介导反义AT1R cDNA转染对人肺动脉平滑肌细胞(PASMCs)增殖的影响。方法: 构建重组反义人AT1R腺病毒(AdCMVahAT1)和表达β-半乳糖苷酶的对照载体AdCMVLacZ，将培养的PASMCs分为DMEM组、ATⅡ组、AdCMVLacZ+ATⅡ组和AdCMVahAT1+ATⅡ组，分别给予相应的干预因素，用RT-PCR和免疫组化检测AT1R的表达，用流式细胞仪检测各组PASMCs的增殖指数。结果:转染病毒后48 h AT1R蛋白表达在AdCMVahAT1组显著低于AdCMVLacZ组和DMEM组。给予ATⅡ刺激48 h，ATⅡ组PASMCs的增殖指数(59.69±3.46)高于DMEM组(50.25±1.34，P<0.01)，转染AdCMVahAT1后再用ATⅡ刺激48 h的AdCMVahAT1+ATⅡ组PASMCs的增殖指数(24.67±3.19)则显著低于3个对照组(P<0.01),而AdCMVLacZ+ATⅡ组(59.53±3.26)和ATⅡ组比较无显著性差异。结论:反义AT1R通过抑制AT1R的表达而抑制PASMCs增殖。

    [关键词] 反义AT1R；肺动脉；平滑肌细胞；增殖

    Influence of Recombinant Adenoviral Vector Containing Human Antisense AT1 Receptor cDNA on Proliferation of Human Pulmonary Artery Smooth Muscle Cells

    XU Da-guo, Zhu Run-qing, TU Ming-li

    (Department of Pathology, Medical College of Wuhan University, Wuhan, Hubei 430071, China； Taihe Hospital, Yunyang Medical College, Shiyan, Hubei 442000, China)

    Abstract: Objective To investigate the influence of human angiotensin Ⅱ (ATⅡ) type 1 receptor (AT1R) antisense cDNA (ahAT1) on proliferation of cultured human pulmonary artery smooth muscle cells (PASMCs). Methods Two recombinant adenoviral vectors, AdCMVahAT1 containing full length antisense cDNA targeting to human AT1R mRNA, and AdCMVLacZ containing LacZ, were constructed by orientation clone and homologous recombination. The PASMCs was divided into 4 groups (DMEM ......