肝肿瘤·白细胞介素1·白细胞介素4·干扰素Ⅱ型·肿瘤转移·肿

原发性肝癌患者细胞因子水平的变化与转移和复发的关系

http://www.100md.com 《中国现代普通外科进展》 2006年第6期

肝肿瘤·白细胞介素1·白细胞介素4·干扰素Ⅱ型·肿瘤转移·肿瘤复发,局部,,肝肿瘤·白细胞介素1·白细胞介素4·干扰素Ⅱ型·肿瘤转移·肿瘤复发，局部,1资料与方法,2结果,3讨论,参考文献

     【摘要】目的：观察原发性肝癌患者细胞因子IL4、IFNγ、IL1β在肝癌组织中的表达，探讨细胞因子的变化、漂移与肝癌转移和复发的关系。方法：应用逆转录聚合酶链反应(RTPCR)检测并比较肝癌组和对照组细胞因子IFNγ(Th1)、IL4(Th2)、IL1β的水平。结果：34例肝细胞性肝癌患者与15例单纯肝硬化患者相比，肝癌组织中IFNγ(Th1)的表达强度、阳性表达频度较对照组明显降低，分别为0.92±0.36(18/34，53.9%)和1.63±0.64(12/15，80.0%)(P<0.01)。IL4(Th2)在肝癌组织中的表达强度较对照组明显增强，分别为1.76±0.49和1.01±0.51(P<0.01)。IL1β在肝癌组织中和对照组的表达强度及频度分别为1.01±0.32(30/34，88.2%)和0.35±0.30(4/15，26.7%)(P<0.001)。3种细胞因子在肝癌组织中有5种不同表达方式。结论：原发性肝癌患者的抗肿瘤免疫存在严重缺陷，细胞因子间的平衡被打破，使已处于抑制状态的Th细胞免疫更趋恶化，致使肿瘤细胞容易发生肝内转移及术后复发。

    【关键词】肝肿瘤·白细胞介素1·白细胞介素4·干扰素Ⅱ型·肿瘤转移·肿瘤复发，局部

    The relationship between immunoregulatory cytokines and intrahepatic metastasis and recurrence in primary hepatocellular carcinoma

    TENG Mujian1,WANG Minghai2,PAN Leyu2

    1Department of Hepatobiliary Surgery of General Surgery Center,Qianfoshan Hospital of Shandong Province(Jinan 250014,China)

    2Medical College of Shandong University(Jinan 250012,China)

    【ABSTRACT】Objective:To observe the expression of immunoregulatory cytokines including interleukin4(IL4,Th2)、 interferonγ(IFNγ,Th1)、interleukinbetal (IL1β) in the tissues of hepatocellular carcinoma(HCC),and to investigate the relationship between the changes of immunoregulatory cytokines in patients with hepatocellular carcinoma and intrahepatic metastasis and recurrence.Methods:The immunoregulatory cytokines:IFNγ(Th1)、IL4(Th2)、IL1β were analyzed by RTPCR.Results:34 cases with HCC were compared with the ones with liver cirrhosis,the expression rates and density of IFNγ(Th1) in the tissues of hepatocellular carcinoma were much lower than that in the liver cirrhosis,they are 0.92±0.36(18/34,53.9%) and 1.63±0.64(12/15,80.0%),respectively.There is significant difference between them (P<0.01).The expression density and levels of IL4(Th2) in the tissues of hepatocellular carcinoma increased clearly,compared with the control group.They are 1.76±0.49 and 1.01±0.51,respectively.Their difference showed statistical significance (P<0.01).The expression rates and levels of IL1β in the tissues of hepatocellular carcinoma and liver cirrhosis were 1.01±0.32(30/34,88.2%) and 0.35±0.30(4/15,26.7%),respectively.There was significant difference between them (P<0.001).Above three immunoregulatory cytokines in patients with hepatocellular carcinoma showed five different expression styles.Conclusion:There is severe deficiency of immune responses against tumors in patients with hepatocellular carcinoma,the balance between those above immunoregulatory cytokines was destroyed,so that immune responses against tumors were weaker and in further suppression,which may enhance the incidence rates of intrahepatic metastasis and recurrence of hepatocellular carcinoma after operation. ......

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