热休克蛋白70对应激性胃溃疡黏膜的保护作用
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王 鹤, 曲 彦, 胡 丹
HSP70;胃黏膜;应激性溃疡,王鹤,曲彦,胡丹,王鹤,通讯作者:,Protectiveroleofheatshockprotein70forgastricmucosawithstressulcer,HeWang,YanQu,DanHu
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王鹤, 青岛大学医学院护理学院 山东省青岛市 266023
曲彦, 胡丹, 青岛大学医学院附属市立医院ICU 山东省青岛市 266011
王鹤, 青岛大学医学院护理学院研究生, 主要从事危重症监护的研究.
通讯作者: 曲彦, 266011, 山东省青岛市, 青岛大学医学院附属市立医院ICU. qdquyan@yahoo.com
电话: 0532-86969701
收稿日期: 2006-11-28 接受日期: 2006-12-27
Protective role of heat shock protein 70 for gastric mucosa with stress ulcer
He Wang, Yan Qu, Dan Hu
He Wang,Nursing School, Qingdao University Medical College, Qingdao 266023, Shandong Province, China
Yan Qu, Dan Hu,Intensive Care Unit, the Affiliated Qingdao Municipal Hospital of Qingdao University Medical College, Qingdao 266011, Shandong Province, China
Correspondence to:Yan Qu, Intensive Care Unit, the Affiliated Qingdao Municipal Hospital of Qingdao University Medical College, Qingdao 266011, Shandong Province, China. qdquyan@yahoo.com
Received:2006-11-28 Accepted:2006-12-27
Abstract
AIM: To investigate whether the expressive intensity of heat shock protein 70 (HSP70) is changed with the progression of stress ulcer.
METHODS: Thirty stress ulcer patients with bleeding and ten patients without bleeding before operation from intensive care unit were included as study subjects. Gastric fluid and blood specimen were collected from these patients. After total RNA was extracted from the shed cells in gastric juice and peripheral white blood cells (PWBC) by TRIzol method, semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to investigate the intensity of HSP70 expression. Meanwhile, immunohistochemistry was used to evaluate the expression of HSP70 in PWBC.
RESULTS: RT-PCR result showed that the expressive intensity of HSP70 in each stage of stress ulcer was remarkably higher than that in the control group (blood specimen: 0.6289 ± 0.1839, 1.1322 ± 0.3683, 0.6855 ± 0.1923 vs 0.3868 ± 0.2071; gastric fluid specimen: 0.7741 ± 0.2442, 1.2385 ± 0.4558, 0.8790 ± 0.2722 vs 0.4626 ± 0.2416; for stage 1, 2, 3 and control group, respectively; all P < 0.05). The expression of HSP70 in stage-2 ulcer was higher than that in stage-1 and -3 one (P < 0.05), but there was no significant difference in the expressive intensity of HSP70 between stage-1 and -3 ulcer (P > 0.05). Immunohistochemistry showed that the level of HSP70 expressive was significantly different among stress ulcer of different stages and the control group (40%, 80%, 30%, 10%, for stage 1, 2, 3 and control group, respectively, χ2 = 10.8383, P < 0.05), and the highest expression level occurred in stage-2 ulcer and the lowest in the control group.
CONCLUSION: HSP70 expression can be detected both in the shed cells in gastric fluid and PWBC from patients with stress ulcer. HSP70 may protect gastric mucosa from stress-induced damage and promote the healing of ulcer.
Key Words: Heat shock proteins 70; Gastric mucosa; Stress ulcer
Wang H, Qu Y, Hu D ......
王鹤, 青岛大学医学院护理学院 山东省青岛市 266023
曲彦, 胡丹, 青岛大学医学院附属市立医院ICU 山东省青岛市 266011
王鹤, 青岛大学医学院护理学院研究生, 主要从事危重症监护的研究.
通讯作者: 曲彦, 266011, 山东省青岛市, 青岛大学医学院附属市立医院ICU. qdquyan@yahoo.com
电话: 0532-86969701
收稿日期: 2006-11-28 接受日期: 2006-12-27
Protective role of heat shock protein 70 for gastric mucosa with stress ulcer
He Wang, Yan Qu, Dan Hu
He Wang,Nursing School, Qingdao University Medical College, Qingdao 266023, Shandong Province, China
Yan Qu, Dan Hu,Intensive Care Unit, the Affiliated Qingdao Municipal Hospital of Qingdao University Medical College, Qingdao 266011, Shandong Province, China
Correspondence to:Yan Qu, Intensive Care Unit, the Affiliated Qingdao Municipal Hospital of Qingdao University Medical College, Qingdao 266011, Shandong Province, China. qdquyan@yahoo.com
Received:2006-11-28 Accepted:2006-12-27
Abstract
AIM: To investigate whether the expressive intensity of heat shock protein 70 (HSP70) is changed with the progression of stress ulcer.
METHODS: Thirty stress ulcer patients with bleeding and ten patients without bleeding before operation from intensive care unit were included as study subjects. Gastric fluid and blood specimen were collected from these patients. After total RNA was extracted from the shed cells in gastric juice and peripheral white blood cells (PWBC) by TRIzol method, semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to investigate the intensity of HSP70 expression. Meanwhile, immunohistochemistry was used to evaluate the expression of HSP70 in PWBC.
RESULTS: RT-PCR result showed that the expressive intensity of HSP70 in each stage of stress ulcer was remarkably higher than that in the control group (blood specimen: 0.6289 ± 0.1839, 1.1322 ± 0.3683, 0.6855 ± 0.1923 vs 0.3868 ± 0.2071; gastric fluid specimen: 0.7741 ± 0.2442, 1.2385 ± 0.4558, 0.8790 ± 0.2722 vs 0.4626 ± 0.2416; for stage 1, 2, 3 and control group, respectively; all P < 0.05). The expression of HSP70 in stage-2 ulcer was higher than that in stage-1 and -3 one (P < 0.05), but there was no significant difference in the expressive intensity of HSP70 between stage-1 and -3 ulcer (P > 0.05). Immunohistochemistry showed that the level of HSP70 expressive was significantly different among stress ulcer of different stages and the control group (40%, 80%, 30%, 10%, for stage 1, 2, 3 and control group, respectively, χ2 = 10.8383, P < 0.05), and the highest expression level occurred in stage-2 ulcer and the lowest in the control group.
CONCLUSION: HSP70 expression can be detected both in the shed cells in gastric fluid and PWBC from patients with stress ulcer. HSP70 may protect gastric mucosa from stress-induced damage and promote the healing of ulcer.
Key Words: Heat shock proteins 70; Gastric mucosa; Stress ulcer
Wang H, Qu Y, Hu D ......
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