重组腺病毒载体介导HOSM基因对肝癌细胞体外增殖的抑制作用
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胡朝全, 孙诚谊, 孙连生, 王玉芝
腺病毒载体;抑瘤素M;基因转染;逆转录聚合酶链反应;肝癌,胡朝全,孙诚谊,孙连生,王玉芝,通讯作者:,Growthsuppressionofhumanhepaticcarcinomacellbyinductionofhumanonc
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胡朝全, 孙诚谊, 贵阳医学院附属医院肝胆外科 贵州省贵阳市 550004
孙连生, 王玉芝, 军事医学科学院放射医学研究所 北京市 100850
贵州省科学技术基金资助项目, No. 20023106
通讯作者: 孙诚谊, 550004, 贵州省贵阳市北京路9号, 贵阳医学院附属医院肝胆外科. scy@public.gz.cn
电话: 0851-86855119-6087 传真: 0851-6820346
收稿日期: 2006-11-19 接受日期: 2006-12-27
Growth suppression of human hepatic carcinoma cell by induction of human oncostatin M gene via recombinant adenovirus
Chao-Quan Hu, Cheng-Yi Sun, Lian-Sheng Sun, Yu-Zhi Wang
Chao-Quan Hu, Cheng-Yi Sun,Department of Hepatobiliary Surgery, the Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou Province, China
Lian-Sheng Sun, Yu-Zhi Wang,Beijing Institute of Radiation Medicine, Beijing 100850, China
Supported bythe Science and Technology Foundation of Guizhou Province, No. 20023106
Correspondence to:Cheng-Yi Sun, Department of Hepatobiliary Surgery, the Affiliated Hospital of Guiyang Medical College, 9 Beijing Road, Guiyang 550004, Guizhou Province, China. scy@public.gz.cn
Received:2006-11-19 Accepted: 2006-12-27
Abstract
AIM: To construct a replication-deficient recombinant adenoviral vector encoding human oncostatin M (HOSM) gene and evaluate its potential for suppressing the cell growth of hepatic carcinoma cell line HepG2 in vitro.
METHODS: Recombinant replication-deficient adenoviral vector encoding human oncostain M (HOSM) gene was constructed using homologous recombination modality. The transfection efficiency of the recombinant virus in HepG2 cells was examined by reporter gene AD-GFP. The expression of exogenous HOSM gene in adenovirus-infected HepG2 cells was identified by reverse transcription polymerase chain reaction (RT-PCR). The proliferation inhibition of HepG2 cells was determined by trypan blue exclusion assay.
RESULTS: The desired recombinant adenovirus vectors were harvested with high titer and purity. The transfection efficiency of the recombinant virus in HepG2 cells was high, and positively correlated with the doses of AD-HOSM. The efficient expression of HOSM gene was detected in AD-HOSM treated cells at the 48th hour, and in vitro growth assay revealed significant growth retardation following AD-HOSM infection.
CONCLUSION: The expression of HOSM delivered by recombinant adenovirus is able to inhibit the proliferation of HepG2 cells in vitro, suggesting that recombinant adenovirus-mediated HOSM gene therapy might be a candidate method for the treatment of hepatic carcinoma.
Key Words: Adenoviral vector; Oncostatin M; Gene transfection; Reverse transcription polymerase chain reaction; Hepatic carcinoma
Hu CQ, Sun CY, Sun LS, Wang YZ. Growth suppression of human hepatic carcinoma cell by induction of human oncostatin M gene via recombinant adenovirus. Shijie Huaren Xiaohua Zazhi 2007;15(7):737-740
摘要
目的: 构建含人抑瘤素M (human oncostatin M, HOSM)基因的复制缺陷型重组腺病毒载体AD-HOSM, 观察其对人肝癌细胞株HepG2在体外生长抑制情况 ......
胡朝全, 孙诚谊, 贵阳医学院附属医院肝胆外科 贵州省贵阳市 550004
孙连生, 王玉芝, 军事医学科学院放射医学研究所 北京市 100850
贵州省科学技术基金资助项目, No. 20023106
通讯作者: 孙诚谊, 550004, 贵州省贵阳市北京路9号, 贵阳医学院附属医院肝胆外科. scy@public.gz.cn
电话: 0851-86855119-6087 传真: 0851-6820346
收稿日期: 2006-11-19 接受日期: 2006-12-27
Growth suppression of human hepatic carcinoma cell by induction of human oncostatin M gene via recombinant adenovirus
Chao-Quan Hu, Cheng-Yi Sun, Lian-Sheng Sun, Yu-Zhi Wang
Chao-Quan Hu, Cheng-Yi Sun,Department of Hepatobiliary Surgery, the Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou Province, China
Lian-Sheng Sun, Yu-Zhi Wang,Beijing Institute of Radiation Medicine, Beijing 100850, China
Supported bythe Science and Technology Foundation of Guizhou Province, No. 20023106
Correspondence to:Cheng-Yi Sun, Department of Hepatobiliary Surgery, the Affiliated Hospital of Guiyang Medical College, 9 Beijing Road, Guiyang 550004, Guizhou Province, China. scy@public.gz.cn
Received:2006-11-19 Accepted: 2006-12-27
Abstract
AIM: To construct a replication-deficient recombinant adenoviral vector encoding human oncostatin M (HOSM) gene and evaluate its potential for suppressing the cell growth of hepatic carcinoma cell line HepG2 in vitro.
METHODS: Recombinant replication-deficient adenoviral vector encoding human oncostain M (HOSM) gene was constructed using homologous recombination modality. The transfection efficiency of the recombinant virus in HepG2 cells was examined by reporter gene AD-GFP. The expression of exogenous HOSM gene in adenovirus-infected HepG2 cells was identified by reverse transcription polymerase chain reaction (RT-PCR). The proliferation inhibition of HepG2 cells was determined by trypan blue exclusion assay.
RESULTS: The desired recombinant adenovirus vectors were harvested with high titer and purity. The transfection efficiency of the recombinant virus in HepG2 cells was high, and positively correlated with the doses of AD-HOSM. The efficient expression of HOSM gene was detected in AD-HOSM treated cells at the 48th hour, and in vitro growth assay revealed significant growth retardation following AD-HOSM infection.
CONCLUSION: The expression of HOSM delivered by recombinant adenovirus is able to inhibit the proliferation of HepG2 cells in vitro, suggesting that recombinant adenovirus-mediated HOSM gene therapy might be a candidate method for the treatment of hepatic carcinoma.
Key Words: Adenoviral vector; Oncostatin M; Gene transfection; Reverse transcription polymerase chain reaction; Hepatic carcinoma
Hu CQ, Sun CY, Sun LS, Wang YZ. Growth suppression of human hepatic carcinoma cell by induction of human oncostatin M gene via recombinant adenovirus. Shijie Huaren Xiaohua Zazhi 2007;15(7):737-740
摘要
目的: 构建含人抑瘤素M (human oncostatin M, HOSM)基因的复制缺陷型重组腺病毒载体AD-HOSM, 观察其对人肝癌细胞株HepG2在体外生长抑制情况 ......
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