转化生长因子β1抗体；肝星状细胞；内皮素；一氧化氮

转化生长因子β1抗体对体外培养肝星状细胞内皮素和一氧化氮的影响

http://www.100md.com 《第四军医大学学报》 2007年第3期

转化生长因子β1抗体；肝星状细胞；内皮素；一氧化氮,,转化生长因子β1抗体；肝星状细胞；内皮素；一氧化氮,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Effects of transforming growth factorβ1 antibody on endothelin and nitric oxide in hepatic stellate cells in vitro

    XIAO YongHong, LIU DianWu, ZHANG Hao

    Department of Epidemiology, Hebei Medical University, Shijiazhuang 050017, China, North China Coal Medical College, Tangshan 063000, China

    【Abstract】 AIM: To study the effects of transforming growth factorβ1 (TGFβ1) antibody on endothelin 1 (ET1) and nitric oxide (NO) in hepatic stellate cells (HSC). METHODS: Suspended liquid of cells at a density of 1×108/L was plated in culture dish under 37℃, 50 mL/L CO2 for 24 h, and divided into groups as follows (4 dishes in each group): control group; TGFβ1 antibody (5-20 mg/L) group, and continuously cultured for 24 h. The culture supernatant was collected. Nitric oxide synthase (NOS) activity was measured using colorimetry, NO level was determined by nitrate reductase technique, and ET1 content in the medium was surveyed by enzyme linked immunosorbant assay (ELISA) method. RESULTS: TGFβ1 antibody markedly inhibited HSC ET1 secretion in a doseeffect manner, and ET1 contents in control group, 10, 15 mg/L TGFβ1 antibody groups were (8.50±0.46), (5.33±0.27) and (3.69±0.33) μg /L respectively, with significant difference in multiple comparison between different dosage groups (P<0.05). Simultaneously, as TGFβ1 antibody dose increased, iNOS activity and NO synthesis increased, which in control group, 10, 15 mg/L TGFβ1 antibody groups were (1.94±0.16), (3.29±0.42), (3.88±0.32) ku/L and (46.75±4.72), (80.68±5.44) ......

百拇医药网 http://www.100md.com/html/DirDu/2007/03/10/38/55/92.htm

您现在查看是摘要页，全文长 8046 字符。