血管生成素-1基因对人胃癌细胞增殖与凋亡的影响
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颜 芳, 欧希龙, 关云艳, 产松苗, 陈国胜, 杨 柳, 孙为豪
腺病毒载体;血管生成素;胃癌;增殖;凋亡颜芳,欧希龙,关云艳,产松苗,陈国胜,杨柳,孙为豪.血管生成素-1基因对人胃癌细胞增殖与凋亡的影响. 世界华人消化杂志2007;15(8)824-830,颜芳,欧希龙,关云艳,杨柳,陈国胜,孙为豪
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颜芳, 欧希龙, 关云艳, 杨柳, 东南大学临床医学院附属中大医院消化科 江苏省南京市 210009
陈国胜, 孙为豪, 南京医科大学第一附属医院老年医学科 江苏省南京市 210029
产松苗, 上海市梅山医院消化内科 江苏省南京市 210039
颜芳, 医学学士, 在读硕士研究生, 主要从事消化系统肿瘤的研究.
通讯作者: 欧希龙, 210009, 江苏省南京市, 东南大学医学院附属中大医院消化科. ouxilong@126.com
电话: 025-83272034
收稿日期: 2006-12-14 接受日期: 2007-01-10
Effect of angiopoietin-1 gene on the proliferation and apoptosis of human gastric cancer cells
Fang Yan, Xi-Long Ou, Yun-Yan Guan, Song-Miao Chan, Guo-Sheng Chen, Liu Yang, Wei-Hao Sun
Fang Yan, Xi-Long Ou, Yun-San Guan, Liu Yang,Department of Gastroenterology, Zhongda Hospital Affiliated to Clinical Medical College of Southeast University, Nanjing 210009, Jiangsu Province, China
Guo-Sheng Chen, Wei-Hao Sun,Department of Geriatrics, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China
Song-Miao Chan,Department of Gastroenterology, Shanghai Meishan Hospital, Nanjing 210039, Jiangsu Province, China
Correspondence to:Xi-Long Ou, Department of Gastroenterology, Zhongda Hospital Affiliated to Clinical Medical College of Southeast University, Nanjing 210009, Jiangsu Province, China. ouxilong@126.com
Received:2006-12-14 Accepted:2007-01-10
AbstractAIM:To explore the effect of angiopoietin-1 (Ang1) gene on the proliferation and apoptosis of human gastric cancer cell line MGC-803.
METHODS: The replication-deficient adenovirus carrying human angiopoietin-1 (Ad-Ang1) and green fluorescent protein (Ad-GFP) were packaged, amplified and purified in human embryonic kidney cells 293 (HEK293). Then gastric cancer cell line MGC-803 was transfected with the resultant adenoviruses. The proliferation and apoptosis of MGC-803 cells were measured by MTT colorimetry and flow cytometry, respectively. The expression of Ang1 and its receptor (Tie-2) were detected by immunohistochemistry.
RESULTS: The recombinant adenovirus vector carrying Ang1 or GFP were amplified and purified successfully with the viral titers of 1.15×1013 and 2.0×1012 PFU/L, respectively. The transfection rate almost reached 100% when the multiplicity of infection (MOI) was 20. The proliferation of MGC-803 cells in Ad-Ang1-transfected group was markedly higher than that in Ad-GFP-transfected group (24 h: t = 16.32, P < 0.0001; 48 h: t = 10.47, P = 0.0005; 72 h: t = 24.59, P < 0.0001) and control group (24 h: t = 10.54, P = 0.0005; 48 h: t = 13.92, P = 0.0002; 72 h: t = 22.46, P < 0.0001). The apoptotic rate of Ag-Ang1-transfected MGC-803 cells was obviously lower than that of Ag-GFP-transfected ones (t = 7.73, P = 0.0015), but there was no difference between Ag-GFP group and control group. The positive rates of Ang1 and Tie-2 expression were significantly higher in Ad-Ang1-transfected cells (Ang-1: 99.2 ± 0.8; Tie-2: 85.4 ± 1.8) than those in the control (Ang-1: 51 ......
颜芳, 欧希龙, 关云艳, 杨柳, 东南大学临床医学院附属中大医院消化科 江苏省南京市 210009
陈国胜, 孙为豪, 南京医科大学第一附属医院老年医学科 江苏省南京市 210029
产松苗, 上海市梅山医院消化内科 江苏省南京市 210039
颜芳, 医学学士, 在读硕士研究生, 主要从事消化系统肿瘤的研究.
通讯作者: 欧希龙, 210009, 江苏省南京市, 东南大学医学院附属中大医院消化科. ouxilong@126.com
电话: 025-83272034
收稿日期: 2006-12-14 接受日期: 2007-01-10
Effect of angiopoietin-1 gene on the proliferation and apoptosis of human gastric cancer cells
Fang Yan, Xi-Long Ou, Yun-Yan Guan, Song-Miao Chan, Guo-Sheng Chen, Liu Yang, Wei-Hao Sun
Fang Yan, Xi-Long Ou, Yun-San Guan, Liu Yang,Department of Gastroenterology, Zhongda Hospital Affiliated to Clinical Medical College of Southeast University, Nanjing 210009, Jiangsu Province, China
Guo-Sheng Chen, Wei-Hao Sun,Department of Geriatrics, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China
Song-Miao Chan,Department of Gastroenterology, Shanghai Meishan Hospital, Nanjing 210039, Jiangsu Province, China
Correspondence to:Xi-Long Ou, Department of Gastroenterology, Zhongda Hospital Affiliated to Clinical Medical College of Southeast University, Nanjing 210009, Jiangsu Province, China. ouxilong@126.com
Received:2006-12-14 Accepted:2007-01-10
AbstractAIM:To explore the effect of angiopoietin-1 (Ang1) gene on the proliferation and apoptosis of human gastric cancer cell line MGC-803.
METHODS: The replication-deficient adenovirus carrying human angiopoietin-1 (Ad-Ang1) and green fluorescent protein (Ad-GFP) were packaged, amplified and purified in human embryonic kidney cells 293 (HEK293). Then gastric cancer cell line MGC-803 was transfected with the resultant adenoviruses. The proliferation and apoptosis of MGC-803 cells were measured by MTT colorimetry and flow cytometry, respectively. The expression of Ang1 and its receptor (Tie-2) were detected by immunohistochemistry.
RESULTS: The recombinant adenovirus vector carrying Ang1 or GFP were amplified and purified successfully with the viral titers of 1.15×1013 and 2.0×1012 PFU/L, respectively. The transfection rate almost reached 100% when the multiplicity of infection (MOI) was 20. The proliferation of MGC-803 cells in Ad-Ang1-transfected group was markedly higher than that in Ad-GFP-transfected group (24 h: t = 16.32, P < 0.0001; 48 h: t = 10.47, P = 0.0005; 72 h: t = 24.59, P < 0.0001) and control group (24 h: t = 10.54, P = 0.0005; 48 h: t = 13.92, P = 0.0002; 72 h: t = 22.46, P < 0.0001). The apoptotic rate of Ag-Ang1-transfected MGC-803 cells was obviously lower than that of Ag-GFP-transfected ones (t = 7.73, P = 0.0015), but there was no difference between Ag-GFP group and control group. The positive rates of Ang1 and Tie-2 expression were significantly higher in Ad-Ang1-transfected cells (Ang-1: 99.2 ± 0.8; Tie-2: 85.4 ± 1.8) than those in the control (Ang-1: 51 ......
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