[摘要] 本文探讨高效液相色谱法测定豆制品中酸性橙Ⅱ和酸性金黄含量的方法，豆制品中酸性金黄Ⅱ经粉碎，提取，过滤，得到的样品提取液用高效液相色谱测定，流动相是甲醇乙酸铵0.02 mol/L=75+25，在检测波长450 nm时，流速1.0 ml/min测定，色谱柱为SymmetryC18(250 mm×4.6 mmid，5 μm)。

    [关键词] 豆制品；酸性金黄Ⅱ；高效液相色谱

    High Performance Liquid Chromatography Determinate of Acid Orange Ⅱ

    and Orarge Bin Bean Sample

    LU Caixia， CHEN Yan, LI Yongbo, et al

    (1.Luonan Disease Prevention Control Central, Luonan, Shaanxi 726100, China;2.Xi'an Disease Prevention Control Central, Xi'an, Shaanxi 710002, China)

    Abstract:A sensitive and accurate High Performance Liquid Chromatography determinate of Acid OrangeⅡand orange B in bean sample.Acid OrangeⅡand orange B were extract from bean sample with the HPLC method was used to analyze estradiel.The optimized detection parameters were as follows:the colum was Symmetry C18 (250 mm×4.6 mmid,5 μm),the mobile phase was methanolaninoacetate (0.02 mmol/ml)=75:25,The phase flow rate was 1.0 ml/min.The UV detector was set at 450 nm.

    Key words:Bean product; Acidity gold; High performance liquid Chromatography

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