高效液相色谱；_阳离子交换柱；_氨苄西林；

HPLC法同时测定注射用氨苄西林钠/氯唑西林钠的含量

http://www.100md.com 《中国抗生素杂志》 2006年第1期

高效液相色谱；,阳离子交换柱；,氨苄西林；,氯唑西林；,含量测定,,高效液相色谱；,阳离子交换柱；,氨苄西林；,氯唑西林；,含量测定,1仪器与试药,2色谱条件,3方法与结果,4讨论,参考文献

     摘要：用阳离子交换柱的HPLC法同时测定注射用氨苄西林钠/氯唑西林钠中氨苄西林和氯唑西林的含量。色谱条件：采用Hypersil SCX柱，磷酸盐缓冲液(001mol/L磷酸氢二铵溶液，用磷酸调节pH值60)∶乙腈(90∶10)为流动相，检测波长225nm。氨苄西林和氯唑西林分别在45～134和44～131μg/ml范围内呈良好的线性关系，日内RSD分别为05%和06%，回收率分别为999%和1000%，含量测定结果与国家药品标准方法所得结果相符。

    关键词：高效液相色谱；阳离子交换柱；氨苄西林；氯唑西林；含量测定

    Simultaneous content determination of ampicillin and cloxacillinin a pharmaceutical formulation for injection by HPLC

    Liu Hao1, Yang Meicheng1,2 and Qiu Shilin1 (1 Shanghai Institute for Drug Control, Shanghai 200233;

    2 School of Pharmacy, Fudan University, Shanghai 200232)

    ABSTRACT A newly proposed HPLC method for simultaneous content determination of ampicillin and cloxacillin in a pharmaceutical formulation for injection was developed. The chromatographic condition was as follows, column: Hypersil SCX; mobile phase: phosphate buffer (001mol/L dibasic ammonium phosphate, adjusted to pH 60 with phosphoric acid)∶acetonitrile (90∶10); detection wavelength: 225nm. The standard curves were linear within the range of 45～134 and 44～131μg/ml for ampicillin and cloxacillin respectively. The precisions in a day (RSD) were 05% and 06% respectively. The assay results complied with those obtained by using the national standard method. ......

百拇医药网 http://www.100md.com/html/DirDu/2007/04/04/41/03/73.htm

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