白细胞介素24；克隆；真核表达；_Ca_Ski细胞_

人宫颈癌Ca Ski细胞IL24基因的克隆鉴定与真核表达

http://www.100md.com 《第四军医大学学报》 2000年第5期

白细胞介素24；克隆；真核表达；,Ca,Ski细胞,,白细胞介素24；克隆；真核表达；,Ca,Ski细胞,人宫颈癌CaSki细胞IL24基因的克隆鉴定与真核表达,【关键词】白细胞介素24；克隆；真核表达；CaSki细胞,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Molecular cloning, identification and eukaryotic expression of IL24 gene in Ca Ski cells

    SHI Hua, CHENG HaiEn, YI FaPing, YANG JunXia, ZHANG Yi, MA YongPing, SONG FangZhou

    Department of Biochemistry and Molecular Biology, Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, Key Laboratory of Clinical Laboratory Diagnostics of Ministry of Education, Basic Medical Institute, Chongqing Medical University, Chongqing 400016, China

    【Abstract】 AIM: To clone human IL24 (hIL24) gene and construct its eukaryotic expression vector, then transfect it into Ca Ski cells to express hIL24 protein. METHODS: hIL24 gene was acquired by RTPCR from the total RNA of Ca Ski cell and cloned into plasmid pcDNA3.1(+) to generate the eukaryotic expression vector named pcDNA3.1(+)hIL24. pcDNA3.1(+)hIL24 was transfected into Ca Ski cells to express hIL24 protein. RESULTS: PCR and sequencing revealed that hIL24 gene was successfully cloned into plasmid pcDNA3.1(+) and its eukaryotic expression vector was constructed. The vector was able to express hIL24 protein after transfected into Ca Ski cells. CONCLUSION: The hIL24 eukaryotic expression vector has been successfully constructed. ......

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