2型糖尿病；胰岛素抵抗；炎症；转化生长因子β1；瘦素；肿瘤坏

炎症细胞因子与实验性2型糖尿病大鼠及肾脏改变

http://www.100md.com 《河北医药》 2007年第2期

2型糖尿病；胰岛素抵抗；炎症；转化生长因子β1；瘦素；肿瘤坏死因子α,,2型糖尿病；胰岛素抵抗；炎症；转化生长因子β1；瘦素；肿瘤坏死因子α,1材料与方法,2结果,3讨论,参考文献

     【摘要】探讨炎性细胞因子与实验性2型糖尿病大鼠及肾脏病变。方法通过高糖高脂饮食结合小剂量链脲佐菌素的方法建立2型糖尿病大鼠动物模型。4、8周末测量大鼠空腹血糖(FBG)、血清胰岛素(FINs)，18周末测量FBG、FINs、转化生长因子β1(TGFβ1)、瘦素(Leptin)、肿瘤坏死因子(TNFα)水平并计算胰岛素抵抗指数(HOMAIR)。用免疫组化、反转录多聚酶链反应(RTPCR)检测TGFβ1在肾脏的表达情况。结果 2型糖尿病大鼠血清TGFβ1、TNFα、Leptin水平明显升高，同时肾脏的TGFβ1表达水平也升高，并伴有肾脏损害。结论实验性2型糖尿病大鼠存在炎性病变及肾脏损害。

    【关键词】 2型糖尿病；胰岛素抵抗；炎症；转化生长因子β1；瘦素；肿瘤坏死因子α

    The changes of inflammatory factors and kidney injury in rat model of type 2 diabetes mellitus

    WANG Zhanjian, XU Huanyu, SU Jieying, et al.

    Department of Endocrinology,The Third Affiliated Hospital of Hebei Medical University, Shijiazhuang,050051

    【Abstract】 Objective To investigate the changes of inflammatory factors and kidney injury in rat model of type 2 diabetes mellitus Methods The model of type 2 diabetes mellitus was set up by feeding highglucose and highfat diet and by injecting STZ into peritoneal cavity of rat. At the end of 4 weeks and 8 weeks, the fasting blood glucose, the levels of fasting serum insulin were measured. At the end of 18 weeks the fasting blood glucose, the levels of fasting serum insulin, serum TGFβ1,TNFα,Leptin in all the groups were measured. Moreover, immunohistochemistry and reverse transcription polymerase chain reaction(RTPCR) was used to examine the pathological change of kidney and expression of TGFβ1.Results The levels of TGFβ1,TNFα,Leptin in the kidney of the rats of type 2 diabetes mellitus obviously increased. The expressive levels of TGFβ1 in the kidney also increased.Conclusions There are inflammatory injuries in the kidney of rat model of type 2 diabetes mellitus. ......

百拇医药网 http://www.100md.com/html/DirDu/2007/04/10/41/83/03.htm

您现在查看是摘要页，全文长 11807 字符。