络石藤；_紫外分光光度法；_总黄酮____

紫外分光光度法测定络石藤中总黄酮含量

http://www.100md.com 《时珍国医国药》 2007年第2期

络石藤；,紫外分光光度法；,总黄酮,,络石藤；,紫外分光光度法；,总黄酮,1器材,2方法与结果[3],3讨论,参考文献：

     摘要：目的对中药络石藤中所含总黄酮的含量进行测定。方法采用紫外分光光度法于500 nm波长处测定。结果总黄酮在8.4～50.4 μg/ml范围内线性关系良好，其回归方程为: A=0.006 714 C +0.070 6(r=0.999 7); 平均加样回收率为96.2% (RSD=1.42%)。结论该方法简便、准确, 可用于络石藤的质量控制。

    关键词：络石藤；紫外分光光度法；总黄酮

    Determination of the Total Flavonoids in Chinese Starjasmine Stem by Spectrophotometry

    ZHOU Qun,FU Haiying, BAI Jiawei, LI Li

    (School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China)

    Abstract：ObjectiveTo establish a method for the determination of the total flavonoids in Chinese Starjasmine Stem. MethodsThe total flavonoids in Chinese Starjasmine Stem was determined by spectrophotometric method at 500 nm wavelength. ResultsSample size showed a good linear relationship at the range of 8.4～50.4 μg/ml, and the curve was A =0.006 714 C+0.070 6(r=0.999 7). The average recovery was 96.2% and RSD was 1.42% (n=6).ConclusionThis method is easy and accurate, and can be used for quality control of Chinese Starjasmine Stem. ......

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