补肾益气颗粒；_补骨脂素；_异补骨脂素；

高效液相色谱法测定补肾益气颗粒中补骨脂素和异补骨脂素含量

http://www.100md.com 《时珍国医国药》 2007年第2期

补肾益气颗粒；,补骨脂素；,异补骨脂素；,高效液相色谱法,,补肾益气颗粒；,补骨脂素；,异补骨脂素；,高效液相色谱法,1器材,2方法与结果,3讨论,参考文献：

     摘要：目的建立补肾益气颗粒中补骨脂素和异补骨脂素的含量测定方法。方法Diamonsil C18柱(200 mm×4.6 mm,5 μm),流动相为甲醇水(55∶45)，流速0.85 ml/min，检测波长245 nm。结果补骨脂素的线性范围为0.08～0.8 μg，r=0.999 98(n=6)，平均加样回收率为99.69%，RSD为2.27%;异补骨脂素的线性范围为0.079 6～0.796 μg，r=0.999 98(n=6)，平均加样回收率为100.25%，RSD为2.82%。结论该法简便，准确，重现性好，可使补骨脂素和异补骨脂素达到基线分离，可以控制补肾益气颗粒的质量。

    关键词：补肾益气颗粒；补骨脂素；异补骨脂素；高效液相色谱法

    Determination of the Psoralen and Isopsorlen in Bushenyiqi Granules by HPLC

    ZHANG Zhuangli, LIU Li, XU Desheng*

    (Shuguang Hospital Affiliated to Shanghai University of TCM, Shanghai 200021,China)

    Abstract：ObjectiveTo establish a method for the determination of psoralen and isopsoralen in Bushenyiqi granules.MethodsThe Diamonsil C18(200 mm×4.6 mm,5 μm)was used.The mobile phase consisted of methanol-water(55:45).The flow rate was 0.85 ml·min-1.The UV detective wavelength was 245 nm.ResultsThe linear range of psoralen was 0.08～0.8 μg, r=0.999 98(n=6) with an average recovery rate of 99.69%(RSD=2.27%),the liner range of isopsoralen was 0.079 6～0.796μg ......

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