何首乌；_高效液相色谱法；_醋酸镁比色法____

炮制方法对何首乌有效成分影响的研究

http://www.100md.com 《时珍国医国药》 2007年第3期

何首乌；,高效液相色谱法；,醋酸镁比色法,,何首乌；,高效液相色谱法；,醋酸镁比色法,1器材,2方法与结果,3讨论,参考文献：

     摘要：目的考察炮制时间对何首乌中有效成分2，3，5，4ˊ四羟基二苯乙烯2OβD葡萄糖苷(简称二苯乙烯苷)和蒽醌类化合物含量的影响。方法何首乌分别采用黑豆汁拌蒸0，2，4，6，8，10 h。HPLC测定炮制饮片中二苯乙烯苷的含量；醋酸镁比色法分别测定炮制饮片中游离蒽醌和总蒽醌的含量。结果用黑豆汁拌蒸的何首乌饮片10 h内二苯乙烯苷含量随炮制时间先逐渐上升而后下降，以6 h为峰；游离蒽醌含量逐渐升高,总蒽醌含量没有显著性变化。结论应规范何首乌饮片的炮制时间。

    关键词：何首乌；高效液相色谱法；醋酸镁比色法

    Influence of Process Methods on Contents of Chemical Component in Radix Polygoni Multiflori

    REN Lijian, JIN Yong, YIN Shouyu*

    (College of Pharmacy, Yanbian University, Yanji 133000,China )

    Abstract：ObjectiveTo investigate the effects of different time of processing Radix Polygonum Mutiflorum on the contents of chemical components 2，3，5，4ˊ-stilbene glucoside and anthraquinone were investigated .MethodsSteamed with black bean sauce for 0,2,4,6,8,10h respectively. HPLC was used to determine the content of 2，3，5，4ˊ-stilbene glucoside. The colorimetry with magnesium acetate as color-developing reagent was applied.The content of free and total anthraquinone were determined. ResultsIn the process of black bean sauce stewing ......

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