Effect of ZeHuangKeLi on aquaporinª²1 expression in lung tissues in diabetic rats

    LI Min1, LI Feng1, HU Bo1, LI Junª²Chang1, ZHANG Yaª²Ping2, WANG Hanª²Min3, ZHANG Sanª²Qi4

    1Department of Traditional Chinese Medicine, 2Department of Endocrinology, 3Department of Nephrology, Xijing Hospital, 4Department of Pharmacy, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM£º To study the expression and activity of aquaporinª²1 (AQPª²1) in pulmonary tissues in diabetic rats after fed ZeHuangKeLi (ZHKL). METHODS£º In 60 SD rats, diabetes mellitus (DM) was induced by highª²glucose and highª²lipid diet and intraperitoneal injection of streptozotocin (STZ). After 2 months, DM rats were chosen and randomly divided into model group, observed group, control group (10 rats per group).After 2 monthª²treatment of ZHKL, we observed the pathologic changes in lungs and detected the expression AQPª²1 by immunohistochemistry. RESULTS£º We observed the thickened basal lamina of pulmonary capillary and increased number of fibres in DM rats. The expression of AQPª²1 decreased in the model group. Compared with the model and control groups, the expression of AQPª²1 increased in the observed group (P<0.05). CONCLUSION£º AQPª²1 is decreased in lung tissues in DMª²induced microcirculation disorder. AQPª²1 plays an important role in lung edema in DM rats. ZHKL can regulate the activity of AQPª²1 to improve the water metabolism of lung tissue. ......