ABSTRACTS OF ORIGINAL ARTICLES
作者：'j, http://www.100md.com
单位：'j, http://www.100md.com
关键词：'j, http://www.100md.com
The production and significance of IL4 in patients with idiopathic nephroctic syndrome Yao Zhi,et al.Department of Immunology,Beijing Medical University,Beijing 100083'j, http://www.100md.com
Samples of serum and PBMC were collected at the same time from 42 pediatric patients with idiopathic nephroctic syndrome (INS) and 28 age-matched healthy individuals.The level os IgE in serum was detected and IL4 in supernatant of PHA-activated PBMC assayed by sandwich ELISA.The level of IL4 and IgE in healthy individuals was 400～500pg/ml and 50～100u/ml respectively,and that of IL4 and Ige in the INS patients was on the average 1 080～4 000pg/ml and 380～1 000U/ml.Positive correlations were noted between the levels of IL4 and IgE,IL4,and quantity of proteinuria in 24 hours in the INS patients.It is suggested that the elevated IgE in the INS patients was induced by the elevated IL4,and that the inbalanced function of T cells and IL4 was contributable to INS.'j, http://www.100md.com
Key words Interleukin Interleukin 2 Nephrotic syndrome Ige
(original article on page 261)q5, http://www.100md.com
The association of Graves' disease with serum soluble interleukin 2 receptor Ren Yuezhong,Song Zuogui.Second Affiliated Hospital,Zhejiang Medical University,Hangzhou 310009q5, http://www.100md.com
The serum soluble interleukin 2 receptor (sIL2R)was measured in 38 first visited patients with Graves' disease and 29 normal controls.The serum sIL2R in 17 patients with Graves' disease was determined after treatment with antithyroid drugs(propylthiouracil)for a short period (1.2±0.5 mouths).The serum sIL2R was measured by sandwich enzyme linked immunosorbent assay.The sIL2R was significantly higher in patients before(3.04±0.19U/ml)and after treatment(2.56±0.41U/ml)than in normal controls (2.20±0.27U/ml,P<0.01).The mean value of serum sIL2R in 17 patients after treatment(2.56±0.41U/ml)was substantially decreased as compared with that before treatment (2.99±0.14U/ml,P<0.01).The serum level of sIL2R in pretreatment patients was correlated significantly with T3(r=0.5032,P<0.05),but was not obviously related to T4or rT3.These findings suggest that the human lymphocytes in patients with Graves' disease were activated in vivo and that sIL2R may be an useful immunological indicator of disease activity.
Key words Receptors,interleukin Goiter,exophthalmic5w-2^, http://www.100md.com
(Original article on page 264)5w-2^, http://www.100md.com
The precursors and effectors of human lymphokine-activated killer(LAK)cells Guo Xi,et al.Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences,Beijing 1007305w-2^, http://www.100md.com
Natural killer cells were enriched in the second fraction (F2) of discontinuous Percoll density gradients from human PBL after centrifugation,whereas T lymphocytes were enriched in F4.The cytotoxicity against K562 by F2 LAK(F2 cells activated by rIL2)was higher than that of F2 cells.F2 LAK also acquired the ability to lyse NK-resistan tumor cell lines and noncultured lung cancer cells(6 out of 9 cases).All these activities of F2 LAK cells were significantly higher than those of F4 LAK cells.Besides,F2 LAK exhibited higher level of lysis against noncultured lung cancer cells than did LAK derived from PBL.Enzyme-labeled immunocytochemistry staining showed that the number of CD+16and CD+8 cells in F2 was much more than that in F4.So the higher lytic ability of F2 LAK might come form two kinds of precursors:CD+16|} typical NK cells and MHC unrestricted CD+8、NK-like' cytotoxic T lymphocytes. The number of CD+4 cells in F4 was much more than that in F2.They shouldn't be the precursors of LAK cells, otherwise the lytic ability of F4 LAK should be higher than that of F2 LAK.
After being cultured with rIL2 for 3 days, F2 cells exhibited proliferative response. But the percentage of CD+16 and CD+8 cells in F2 LAK did not change much, and remained much higher than that in F4 LAK. So the effector cells of short-term cultured LAK might be the activated CD+16 NK cells and CD+8、 NK-like' cytotoxic T lymphocytes as well. It is expected that LAK cells generated by culturing purfied CD+16 and MHC unrestricted CD+8 cells instead of PBL with rIL2 may achieve greater anti-tumor therapeutic effcet.k63{ie, 百拇医药
Key words Natural killer cells Lymphokine-actiuated killer cellsk63{ie, 百拇医药
(Original article on page 266)k63{ie, 百拇医药
Types of anticellular structure antisera in scleroderma patients Ni Zumei,et al.Shanghai Institute of Cellbiology,Chinese Academy of Sciences,Shanghai 200031.k63{ie, 百拇医药
The sera of 338 scleroderma patients were cytologically examined with indirect immuno-fluorescence method.The sera were classified according to the cellular structure,to shich they were against respectively.Among these sera,six main types of cellular structure antiserum were found:antikinetochore,antinucleolus,antichromatin,antihetrochromatin,antichromosome and anticentrosome.Some of these main types can further be subtyped with respect to the substructure or the chemical comnonents(antigens)of a particular cellular structure.These types of antiserum may possibly be correlated with the clinical signs of scleroderma patients.For exploring the possibility to use for diagnosis.
Key words Scleroderma Autoantibody Antigen%!/?, http://www.100md.com
(Original article on page 270)%!/?, http://www.100md.com
Synthetic oligopeptide of HDAg and its clinical application Zheng Hong,et al,Department of Infectious Diseases,Southwest Hospital,Third Military Medical College,Chongqing 360038.%!/?, http://www.100md.com
A 27-peptide fragment derived from natural HDAg sequence was selected,identified and synthesized.It was used to develop an EIA method for detection of anti-HD.The 27-peptide possessed the similar antigenicity with the corresponding fragment of natural HDAg,and could also compete with natural HDAg for serum anti-HD.High specificity and no cross-reaction were found among the peptide and normal human sera,normal mice sera and sera positive with HAV,HBV or HCV antibody alone.36 serum samples were identified previously,with Abbott kits with a coincident rate of 97.2%.Anti-HD was also detected in serum samples of some blood donors and patients with liver diseases and HBV infection from 1990～1992. One(0.33%) of 300 blood donors was positive (two times higher than normal ALT level in serum).62 patients with hepatitis A and 58 patients with non-hepatitis B were anti-HD negative.100(11.64%)of 859 patients with HBV infection were positive:ASC 13/410(3.17%),AH 7/63(10.29%),CPH 1/9(11.11%),CAH 22/121(18.18%),SH 15/75(20.00%),LC 23/78(29.49%),PHC 19/89(19.39%).The results are consisten with our previous reports.
Key words Peptide fragments Hepatitis D virus Hepatitis D virus antigens+]?r), http://www.100md.com
(Original article on page 273)+]?r), http://www.100md.com
Protective action of taurine on ischemia-reperfusion liver injury in rats and its mechanism Chen Yuexiang,et al.Department of Gastroenterology,Tangdu Hospital,Fourth Military Medical University,Xi'an 710038+]?r), http://www.100md.com
The protective effect of taurine on ischemiareperfusion damages of rat perfused liver and its possible mechanism were investigated.Reflow with 20mmol/L taurine markedly ameliorated reperfusion injury of post-ischemic liver as evidenced by reduction of lipid peroxidation (LPO),mitochondrial calcium overload in hepatocytes,as well as leakage of intracellular LDH and protein.Furthermore,taurine could significantly supress in vitro LPO formation of hepatocyte plasma membrane induced by generating system of free redicals,inhibite calcium uptake andpromote calciun release by mitochondria in addition to a membrane stabilizing action on rat isolated hepatic lysosome. The results demonstrated that taurine possesses significant protective efficacy on ischemia-reperfusion liver damage,and that the mechanism of its anti-hepatic injury may be mainly related to inhibiting LPO formation,regulating cellular calcium homeostasis and stabilizing biological membrane.
Key words Amino acids,diamino Liver hemoperfusion Lipid peroxides)x9kljp, http://www.100md.com
(Original article on page 276))x9kljp, http://www.100md.com
Electrochemical analysis in differential diagnosis of benigh and malignant oral maxillofacial tumors Zhang chunyang,et al.First Teaching Hospital,Harbin Medical University,Harbin 150001)x9kljp, http://www.100md.com
A bioelectrochemical-sensor device was designed according to the priniciples of galvanic cell reaction.Modified bioelectrochemically,the device was used to measure the curren of samples from benign and malignant tumor tissues.Statistical analysis and clinical test showed that the current values(426 μA) of the malignant tissue were higher than those(216 μA) of the benign tissue.The sensitivity and specificity were 95% and 90% respectively.This method provided important information about benignancy or malignancy of the tumor and its involvement and metastasis.)x9kljp, http://www.100md.com
Key words Electrochemistry Neoplasms,maxillofacial Diagnosis,rapid)x9kljp, http://www.100md.com
单位：'j, http://www.100md.com
关键词：'j, http://www.100md.com
The production and significance of IL4 in patients with idiopathic nephroctic syndrome Yao Zhi,et al.Department of Immunology,Beijing Medical University,Beijing 100083'j, http://www.100md.com
Samples of serum and PBMC were collected at the same time from 42 pediatric patients with idiopathic nephroctic syndrome (INS) and 28 age-matched healthy individuals.The level os IgE in serum was detected and IL4 in supernatant of PHA-activated PBMC assayed by sandwich ELISA.The level of IL4 and IgE in healthy individuals was 400～500pg/ml and 50～100u/ml respectively,and that of IL4 and Ige in the INS patients was on the average 1 080～4 000pg/ml and 380～1 000U/ml.Positive correlations were noted between the levels of IL4 and IgE,IL4,and quantity of proteinuria in 24 hours in the INS patients.It is suggested that the elevated IgE in the INS patients was induced by the elevated IL4,and that the inbalanced function of T cells and IL4 was contributable to INS.'j, http://www.100md.com
Key words Interleukin Interleukin 2 Nephrotic syndrome Ige
(original article on page 261)q5, http://www.100md.com
The association of Graves' disease with serum soluble interleukin 2 receptor Ren Yuezhong,Song Zuogui.Second Affiliated Hospital,Zhejiang Medical University,Hangzhou 310009q5, http://www.100md.com
The serum soluble interleukin 2 receptor (sIL2R)was measured in 38 first visited patients with Graves' disease and 29 normal controls.The serum sIL2R in 17 patients with Graves' disease was determined after treatment with antithyroid drugs(propylthiouracil)for a short period (1.2±0.5 mouths).The serum sIL2R was measured by sandwich enzyme linked immunosorbent assay.The sIL2R was significantly higher in patients before(3.04±0.19U/ml)and after treatment(2.56±0.41U/ml)than in normal controls (2.20±0.27U/ml,P<0.01).The mean value of serum sIL2R in 17 patients after treatment(2.56±0.41U/ml)was substantially decreased as compared with that before treatment (2.99±0.14U/ml,P<0.01).The serum level of sIL2R in pretreatment patients was correlated significantly with T3(r=0.5032,P<0.05),but was not obviously related to T4or rT3.These findings suggest that the human lymphocytes in patients with Graves' disease were activated in vivo and that sIL2R may be an useful immunological indicator of disease activity.
Key words Receptors,interleukin Goiter,exophthalmic5w-2^, http://www.100md.com
(Original article on page 264)5w-2^, http://www.100md.com
The precursors and effectors of human lymphokine-activated killer(LAK)cells Guo Xi,et al.Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences,Beijing 1007305w-2^, http://www.100md.com
Natural killer cells were enriched in the second fraction (F2) of discontinuous Percoll density gradients from human PBL after centrifugation,whereas T lymphocytes were enriched in F4.The cytotoxicity against K562 by F2 LAK(F2 cells activated by rIL2)was higher than that of F2 cells.F2 LAK also acquired the ability to lyse NK-resistan tumor cell lines and noncultured lung cancer cells(6 out of 9 cases).All these activities of F2 LAK cells were significantly higher than those of F4 LAK cells.Besides,F2 LAK exhibited higher level of lysis against noncultured lung cancer cells than did LAK derived from PBL.Enzyme-labeled immunocytochemistry staining showed that the number of CD+16and CD+8 cells in F2 was much more than that in F4.So the higher lytic ability of F2 LAK might come form two kinds of precursors:CD+16|} typical NK cells and MHC unrestricted CD+8、NK-like' cytotoxic T lymphocytes. The number of CD+4 cells in F4 was much more than that in F2.They shouldn't be the precursors of LAK cells, otherwise the lytic ability of F4 LAK should be higher than that of F2 LAK.
After being cultured with rIL2 for 3 days, F2 cells exhibited proliferative response. But the percentage of CD+16 and CD+8 cells in F2 LAK did not change much, and remained much higher than that in F4 LAK. So the effector cells of short-term cultured LAK might be the activated CD+16 NK cells and CD+8、 NK-like' cytotoxic T lymphocytes as well. It is expected that LAK cells generated by culturing purfied CD+16 and MHC unrestricted CD+8 cells instead of PBL with rIL2 may achieve greater anti-tumor therapeutic effcet.k63{ie, 百拇医药
Key words Natural killer cells Lymphokine-actiuated killer cellsk63{ie, 百拇医药
(Original article on page 266)k63{ie, 百拇医药
Types of anticellular structure antisera in scleroderma patients Ni Zumei,et al.Shanghai Institute of Cellbiology,Chinese Academy of Sciences,Shanghai 200031.k63{ie, 百拇医药
The sera of 338 scleroderma patients were cytologically examined with indirect immuno-fluorescence method.The sera were classified according to the cellular structure,to shich they were against respectively.Among these sera,six main types of cellular structure antiserum were found:antikinetochore,antinucleolus,antichromatin,antihetrochromatin,antichromosome and anticentrosome.Some of these main types can further be subtyped with respect to the substructure or the chemical comnonents(antigens)of a particular cellular structure.These types of antiserum may possibly be correlated with the clinical signs of scleroderma patients.For exploring the possibility to use for diagnosis.
Key words Scleroderma Autoantibody Antigen%!/?, http://www.100md.com
(Original article on page 270)%!/?, http://www.100md.com
Synthetic oligopeptide of HDAg and its clinical application Zheng Hong,et al,Department of Infectious Diseases,Southwest Hospital,Third Military Medical College,Chongqing 360038.%!/?, http://www.100md.com
A 27-peptide fragment derived from natural HDAg sequence was selected,identified and synthesized.It was used to develop an EIA method for detection of anti-HD.The 27-peptide possessed the similar antigenicity with the corresponding fragment of natural HDAg,and could also compete with natural HDAg for serum anti-HD.High specificity and no cross-reaction were found among the peptide and normal human sera,normal mice sera and sera positive with HAV,HBV or HCV antibody alone.36 serum samples were identified previously,with Abbott kits with a coincident rate of 97.2%.Anti-HD was also detected in serum samples of some blood donors and patients with liver diseases and HBV infection from 1990～1992. One(0.33%) of 300 blood donors was positive (two times higher than normal ALT level in serum).62 patients with hepatitis A and 58 patients with non-hepatitis B were anti-HD negative.100(11.64%)of 859 patients with HBV infection were positive:ASC 13/410(3.17%),AH 7/63(10.29%),CPH 1/9(11.11%),CAH 22/121(18.18%),SH 15/75(20.00%),LC 23/78(29.49%),PHC 19/89(19.39%).The results are consisten with our previous reports.
Key words Peptide fragments Hepatitis D virus Hepatitis D virus antigens+]?r), http://www.100md.com
(Original article on page 273)+]?r), http://www.100md.com
Protective action of taurine on ischemia-reperfusion liver injury in rats and its mechanism Chen Yuexiang,et al.Department of Gastroenterology,Tangdu Hospital,Fourth Military Medical University,Xi'an 710038+]?r), http://www.100md.com
The protective effect of taurine on ischemiareperfusion damages of rat perfused liver and its possible mechanism were investigated.Reflow with 20mmol/L taurine markedly ameliorated reperfusion injury of post-ischemic liver as evidenced by reduction of lipid peroxidation (LPO),mitochondrial calcium overload in hepatocytes,as well as leakage of intracellular LDH and protein.Furthermore,taurine could significantly supress in vitro LPO formation of hepatocyte plasma membrane induced by generating system of free redicals,inhibite calcium uptake andpromote calciun release by mitochondria in addition to a membrane stabilizing action on rat isolated hepatic lysosome. The results demonstrated that taurine possesses significant protective efficacy on ischemia-reperfusion liver damage,and that the mechanism of its anti-hepatic injury may be mainly related to inhibiting LPO formation,regulating cellular calcium homeostasis and stabilizing biological membrane.
Key words Amino acids,diamino Liver hemoperfusion Lipid peroxides)x9kljp, http://www.100md.com
(Original article on page 276))x9kljp, http://www.100md.com
Electrochemical analysis in differential diagnosis of benigh and malignant oral maxillofacial tumors Zhang chunyang,et al.First Teaching Hospital,Harbin Medical University,Harbin 150001)x9kljp, http://www.100md.com
A bioelectrochemical-sensor device was designed according to the priniciples of galvanic cell reaction.Modified bioelectrochemically,the device was used to measure the curren of samples from benign and malignant tumor tissues.Statistical analysis and clinical test showed that the current values(426 μA) of the malignant tissue were higher than those(216 μA) of the benign tissue.The sensitivity and specificity were 95% and 90% respectively.This method provided important information about benignancy or malignancy of the tumor and its involvement and metastasis.)x9kljp, http://www.100md.com
Key words Electrochemistry Neoplasms,maxillofacial Diagnosis,rapid)x9kljp, http://www.100md.com