三株散发性戊型肝炎病毒变异株的部分序列比较
作者：杨军 张华远 王佑春 毛群颖 李河民
单位：中国药品生物制品检定所，北京 100050
关键词：戊型肝炎病毒；核苷酸同源性；进化树
中国病毒学000305摘要：对三株散发性戊型肝炎病毒Ch-T11、Ch-T21、Ch-T40的部分基因组做克隆测序，经比较发现与其它国内外HEV株ORF2部分相应核苷酸序列同源性在78.3-81.3%，Ch-T21与Ch-T40的核苷酸同源性为98.8%，而Ch-T11与前两者的同源性则分别为89.8%和90.2%。Ch-T11、Ch-T21、Ch-T40与其它HEV株相应氨基酸序列同源性在95.8-97.9%，它们之间的氨基酸同源性则为100%。系统进化树分析显示，这三株HEV可能代表着一新型HEV。
中图分类号：R373.21，R512.6 文献标识码：A
, http://www.100md.com
文章编号：1003-5125(2000)03-0232-05
Partial sequence comparison of three sporadic hepatitis E variants
YANG Jun，ZHANG Hua-yuan，WANG You-chun，MAO Qun-ying，LI He-min
(National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China)
Abstract: Partial genomes of three strains of sporadic hepatitis E virus (Ch-T11,Ch-T21,Ch-T40) were cloned and sequenced. The nucleotide and putative amino acid homology of Ch-T11,Ch-T21,Ch-T40 and other HEV strains were 78.3-81.3% and 95.8-97.9%, respectively. The nucleotide homology between Ch-T21 and Ch-T40 was 98.8%, while the nucleotide homology of Ch-T11 and Ch-T21,Ch-T40 was 89.8% and 90.2%, respectively. The amino acid homology among Ch-T11,Ch-T21 and Ch-T40 was 100%. Phylogenetic analysis suggested that Ch-T11, Ch-T21 and Ch-T40 may represent a new genotype of HEV.
, 百拇医药
Key words: Hepatitis E virus; Nucleotide homology, Phylogenetic tree
作者简介：杨军(1969年-)，男，山东青岛人，助研，中国药品生物制品检定所硕士
毕业，从事乙肝、戊肝的分子生物学研究、诊断试剂质控参考品研制及质量控制。
参考文献
[1] Wang YC, Ling R, Erker JC, et al. A divergent genotype of hepatitis E virus in Chinese patients with accute hepatitis [J]. J Gen Virol, 1999,80:169-177.
[2] Li K, Zhuang H, Zhu W. Partial nucleotide sequencing of hepatitis E viruses isolated from 14 cities of China: Identification of 2 major variants of hepatitis E virus [J]. J Med Virol, In press
, 百拇医药
[3] Huang RT, Nakazono N, Ishii K et al. Existing variations on the gene structure of hepatitis E virus strains from some regions of China [J]. J Med Virol, 1995,47:303-308.
[4] Hsieh SY, Yang PY, Ho YP, et al. Identification of a novel hepatitis E virus responsible for sporadic acute hepatitis in Taiwan. J Med Virol, 1998,55:300-304.
[5] Schlauder GG, Dawson GJ, Erker JC et al. The sequence and phylogenetic analysis of a novel hepatitis E virus isolated from a patient with acute hepatitis reported in the United States [J]. J Gen Virol, 1998,79:447-456.
收稿日期：1999-05-10，修回日期：1999-07-05, 百拇医药
单位：中国药品生物制品检定所，北京 100050
关键词：戊型肝炎病毒；核苷酸同源性；进化树
中国病毒学000305摘要：对三株散发性戊型肝炎病毒Ch-T11、Ch-T21、Ch-T40的部分基因组做克隆测序，经比较发现与其它国内外HEV株ORF2部分相应核苷酸序列同源性在78.3-81.3%，Ch-T21与Ch-T40的核苷酸同源性为98.8%，而Ch-T11与前两者的同源性则分别为89.8%和90.2%。Ch-T11、Ch-T21、Ch-T40与其它HEV株相应氨基酸序列同源性在95.8-97.9%，它们之间的氨基酸同源性则为100%。系统进化树分析显示，这三株HEV可能代表着一新型HEV。
中图分类号：R373.21，R512.6 文献标识码：A
, http://www.100md.com
文章编号：1003-5125(2000)03-0232-05
Partial sequence comparison of three sporadic hepatitis E variants
YANG Jun，ZHANG Hua-yuan，WANG You-chun，MAO Qun-ying，LI He-min
(National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China)
Abstract: Partial genomes of three strains of sporadic hepatitis E virus (Ch-T11,Ch-T21,Ch-T40) were cloned and sequenced. The nucleotide and putative amino acid homology of Ch-T11,Ch-T21,Ch-T40 and other HEV strains were 78.3-81.3% and 95.8-97.9%, respectively. The nucleotide homology between Ch-T21 and Ch-T40 was 98.8%, while the nucleotide homology of Ch-T11 and Ch-T21,Ch-T40 was 89.8% and 90.2%, respectively. The amino acid homology among Ch-T11,Ch-T21 and Ch-T40 was 100%. Phylogenetic analysis suggested that Ch-T11, Ch-T21 and Ch-T40 may represent a new genotype of HEV.
, 百拇医药
Key words: Hepatitis E virus; Nucleotide homology, Phylogenetic tree
作者简介：杨军(1969年-)，男，山东青岛人，助研，中国药品生物制品检定所硕士
毕业，从事乙肝、戊肝的分子生物学研究、诊断试剂质控参考品研制及质量控制。
参考文献
[1] Wang YC, Ling R, Erker JC, et al. A divergent genotype of hepatitis E virus in Chinese patients with accute hepatitis [J]. J Gen Virol, 1999,80:169-177.
[2] Li K, Zhuang H, Zhu W. Partial nucleotide sequencing of hepatitis E viruses isolated from 14 cities of China: Identification of 2 major variants of hepatitis E virus [J]. J Med Virol, In press
, 百拇医药
[3] Huang RT, Nakazono N, Ishii K et al. Existing variations on the gene structure of hepatitis E virus strains from some regions of China [J]. J Med Virol, 1995,47:303-308.
[4] Hsieh SY, Yang PY, Ho YP, et al. Identification of a novel hepatitis E virus responsible for sporadic acute hepatitis in Taiwan. J Med Virol, 1998,55:300-304.
[5] Schlauder GG, Dawson GJ, Erker JC et al. The sequence and phylogenetic analysis of a novel hepatitis E virus isolated from a patient with acute hepatitis reported in the United States [J]. J Gen Virol, 1998,79:447-456.
收稿日期：1999-05-10，修回日期：1999-07-05, 百拇医药