内毒素血症大鼠肠道传输改变及一氧化氮作用
作者：史洪涛 冷恩仁 桂先勇
单位：第三军医大学大坪医院消化科(400042)
关键词：内毒素血症；肠道传输；一氧化氮
重庆医学000409摘 要：目的探讨内毒素血症状态下肠道传输改变及一氧化氮变化，为多器官功能衰竭的发生提供理论依据。方法104只雄性Wistar大鼠，随机分为对照组和内毒素血症组：肠道传输速度研究采用腹腔注射LPS10mg/10kg；观察内毒素血症3、6、12小时后大鼠血浆NO含量及空肠组织一氧化氮合酶(NOS)活性(包括iNOS，cNOS)变化，小肠铺片技术显示NOS阳性肌间神经丛，以及空肠免疫组化染色显示NOSⅡ阳性神经在肠道分布及内毒素血症后的变化。结果内毒素血症后小肠传输速度加快，内毒素血症3、6、12小时血浆ND、空肠NOS活性(iNOS.cNOS)增强，并呈上升趋势，与对照组比较相差显著(P<0.01)，肌间神经丛及空肠肠壁NOS阳性神经纤维及产物表达增多，与空肠组织含量相一致。结论内毒素血症后肠道传输功能变化可能与NO有关。
, http://www.100md.com
Reasearch of the Effect of Nitric Oxide on the Change of Intestinal Transit in Rats With Endofoxemia
Shi Hongtao ,Leng Enren ,Gui Xianyong
(Department of Gastroenterology,Daping Hospital,The Thind Military Medical University.Chongqing 400042)
Abstract：Aim: To study the effect of nitric oxide (NO) on the change of intestinal transit during endotoxemia and to provide a basis for the mechanism of endotoxin translocation and multiple organ failure(MOF). Methods:The Wistar rats were divided into 2 groups: normal control and.endotoxemia group which were given LPS 10mg/kg intraperitonealy;Tissue levels of nitric oxide synthase(NOS) activity including constitude NOS (cNOS) and inducible NOS(iNOS) and serum levels of NO were measured at 3, 6, 12 hours postendotoxemia(to give IPS 10mg/kg intravenously). Histochemical and immohistochemical methods were used to evaluate the effect of endotoxemia on distribution of NOS immunoreactivity positive nerve fibre and product in jejuna. Results:Intestinal transit was rapid during endotoxemia;The tissue levels of NOS activity increased markdly on postendotoxemia.Increased NOS immunoreactive sfowed similar alteration with the tissue and serum levels of NO. Conclusion;The change of intesinal transit during endotoxemnia possibly is asscoiated with NO.
, 百拇医药
Keywords：Endotoxemia; Intestinal;transit;NO
参考文献：
[1]Akexabder JW, Buyce ST, Babcock GF, et al.The process of microbial uanslocation. Ann Surg, 1990, 212: 496
[2]Hegesh E, Shiloah J. Blood niuates and infantile hemoglobinemia.Clin Chim Acta, 1983,125:107
[3]Wirthlin DJ, Cullen JJ, Spates ST, et al. Gastrointestinal transit during endotoxima:the role of nitric oxide. J Surg Res, 1996, 60:307
, http://www.100md.com
[4]Costa M, Buffa R, Fumess JB, et al. Immunohistochemical localization of polypeptides in peripheral autonomic nerves using whole mount preparation. Histochemistry, 1980, 65(2):157
[5]Vanderwinden JM, De Leaf MH, Schiffmann SN, et al. Nitric oxidesynthase distribution in the enteric nervous system of Hischsprung’s disease. Gastroenterology, 1993,105:969
[6]Stark ME, Szurszewski JH, Role of nitric oxide in gastrointestinal and hepatic function and disease. Gastroenterology, 1992,103:1928
[7]Weisbrodt NW, Pressley TA, Li YF, et al. Decreased ileal musclecontractility and increased NOS Ⅱ expression induced by lipopolysaccharide. Am J Physiol, 1996, 271: G454, 百拇医药
单位：第三军医大学大坪医院消化科(400042)
关键词：内毒素血症；肠道传输；一氧化氮
重庆医学000409摘 要：目的探讨内毒素血症状态下肠道传输改变及一氧化氮变化，为多器官功能衰竭的发生提供理论依据。方法104只雄性Wistar大鼠，随机分为对照组和内毒素血症组：肠道传输速度研究采用腹腔注射LPS10mg/10kg；观察内毒素血症3、6、12小时后大鼠血浆NO含量及空肠组织一氧化氮合酶(NOS)活性(包括iNOS，cNOS)变化，小肠铺片技术显示NOS阳性肌间神经丛，以及空肠免疫组化染色显示NOSⅡ阳性神经在肠道分布及内毒素血症后的变化。结果内毒素血症后小肠传输速度加快，内毒素血症3、6、12小时血浆ND、空肠NOS活性(iNOS.cNOS)增强，并呈上升趋势，与对照组比较相差显著(P<0.01)，肌间神经丛及空肠肠壁NOS阳性神经纤维及产物表达增多，与空肠组织含量相一致。结论内毒素血症后肠道传输功能变化可能与NO有关。
, http://www.100md.com
Reasearch of the Effect of Nitric Oxide on the Change of Intestinal Transit in Rats With Endofoxemia
Shi Hongtao ,Leng Enren ,Gui Xianyong
(Department of Gastroenterology,Daping Hospital,The Thind Military Medical University.Chongqing 400042)
Abstract：Aim: To study the effect of nitric oxide (NO) on the change of intestinal transit during endotoxemia and to provide a basis for the mechanism of endotoxin translocation and multiple organ failure(MOF). Methods:The Wistar rats were divided into 2 groups: normal control and.endotoxemia group which were given LPS 10mg/kg intraperitonealy;Tissue levels of nitric oxide synthase(NOS) activity including constitude NOS (cNOS) and inducible NOS(iNOS) and serum levels of NO were measured at 3, 6, 12 hours postendotoxemia(to give IPS 10mg/kg intravenously). Histochemical and immohistochemical methods were used to evaluate the effect of endotoxemia on distribution of NOS immunoreactivity positive nerve fibre and product in jejuna. Results:Intestinal transit was rapid during endotoxemia;The tissue levels of NOS activity increased markdly on postendotoxemia.Increased NOS immunoreactive sfowed similar alteration with the tissue and serum levels of NO. Conclusion;The change of intesinal transit during endotoxemnia possibly is asscoiated with NO.
, 百拇医药
Keywords：Endotoxemia; Intestinal;transit;NO
参考文献：
[1]Akexabder JW, Buyce ST, Babcock GF, et al.The process of microbial uanslocation. Ann Surg, 1990, 212: 496
[2]Hegesh E, Shiloah J. Blood niuates and infantile hemoglobinemia.Clin Chim Acta, 1983,125:107
[3]Wirthlin DJ, Cullen JJ, Spates ST, et al. Gastrointestinal transit during endotoxima:the role of nitric oxide. J Surg Res, 1996, 60:307
, http://www.100md.com
[4]Costa M, Buffa R, Fumess JB, et al. Immunohistochemical localization of polypeptides in peripheral autonomic nerves using whole mount preparation. Histochemistry, 1980, 65(2):157
[5]Vanderwinden JM, De Leaf MH, Schiffmann SN, et al. Nitric oxidesynthase distribution in the enteric nervous system of Hischsprung’s disease. Gastroenterology, 1993,105:969
[6]Stark ME, Szurszewski JH, Role of nitric oxide in gastrointestinal and hepatic function and disease. Gastroenterology, 1992,103:1928
[7]Weisbrodt NW, Pressley TA, Li YF, et al. Decreased ileal musclecontractility and increased NOS Ⅱ expression induced by lipopolysaccharide. Am J Physiol, 1996, 271: G454, 百拇医药