Irbesartan 和培哚普利对压力超负荷大鼠循环与心脏组织 RAS 的影响
作者：张萍 何国祥 迟路湘 王国超
单位：第三军医大学西南医院心内科(400038)
关键词：压力超负荷；肾素-血管紧张素系统；循环；心脏局部组织
重庆医学000410摘 要：目的旨在研究血管紧张转换酶(ACE)抑制剂培哚普利和血管紧张素Ⅱ受体的Ⅰ型受体(AT1R)拮抗剂Irbesartan对循环和心脏局部组织肾素-血管紧张素系统(RAS)影响。方法采用大鼠腹主动脉缩窄模型，用放射免疫法测定血浆肾素(RA)浓度、血管紧张素Ⅱ(AngⅡ)含量，心肌RA浓度、AngⅡ含量；比色法测血清和心肌ACE活性。结果循环AngⅡ含量、ACE活性与LVMⅠ之间均无相关性(r1=0.0967，r2=0.1450，P>0.05)。Irbestarsan组循环中RA、AngⅡ含量和ACE活性显著高于手术组(P<0.01)；培哚普利组循环RA显著高于手术组(P<0.01)，ACE活性和AngⅡ含量显著低于手术组(P<0.01)。手术组心肌AngⅡ含量、ACE活性均与LVMⅠ呈显著正相关(r1=0.8266，r2=0.828P<0.01)。Irbesartan组心肌RA、AngⅡ和ACE均显著低予手术组(P<0.01)；培哚普利组心肌RA显著高于手术组(P<0.05)，但ACE活性和AngⅡ含量显著低予手术组(P<0.01)。结论心脏局部组织RAS而非循环RAS在压力超负荷致心肌肥大中起重要作用，培哚普利和Irbesartan防止心肌肥厚发生的重要机制之一是阻断心脏组织RAS，抑制心肌组织局部AngⅡ的生成。
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Effects of Irbesartan and Peridopril on Circulating and Local Renin-Angiotensin System(RAS) in Rats With Pressure-over-Loading Cardiac Hypertrophy
Zhang Ping ,He Guoxiang ,Chi Luxiang
(Department of Cardiology,Southwest Hospital,Third Military Madical University,Chongqing 400038)
Abstract：Aim:To investigate the effects of Irbesartan and peridopril on circulating and local renin-angiiotension system (RAS)in rats.Methods:Male wistar rats were divided randomly into 4 group:shame-operated(So), abdominal aorta constiction(AC), AC with Irbesartan(AC+ Irb), AC with Peridopril (AC+Per). At 10th week after operation, circulating and ventricular renin activity (RA), angiotensin Ⅱ(Ang Ⅱ), angiotensin I converting enzyme(ACE) activity were measured respectively by radioimmunoassay(RIA) and biochemical analysis. Results:Levels of circulating RA, Ang Ⅱ and ACE in AC+Irb were significantly higher than those in AC (P<0.01), Plasma RA in AC+Per was significantly higher than that in AC(P<0.01 ), plasma Ang Ⅱ and serum ACE were significantly lover than those in AC(0.01). Components of cardiac RAS in AC+Irb were significantly lower than those in AC P<0.05). Compared with AC, ventricular RA in AC+Per was decreased obviously(p<0.01),ACE and Ang Ⅱ were increased obviously(P<0.01).Left ventricular mass index(LVMⅠ) was positivily correlated with both ventricular ACE and Ang Ⅱ(r1=0.8266, r2=0.828 P<0.01), not with circulating ACE and Ang Ⅱ. Conclusion: These results suggest that cardiac RAS plays an important role in the cardiac hypertrophy during pressure over-loading. A machanism of preventing cardiac hypertrophy by Irbesartan and Peridopril is due to blocking cardiac RAS, inhibiting production of ventricular Ang Ⅱ.
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Keywords：Pressure-overload Renin-angiotensin system Circulation Cardiac local tissue
参考文献：
[1]Danser AH.Local renin-angiotensin system.Mol Cell Biochem，1996，167(3)：211
[2]Zhang x，Dostal DE，Reiss K，et al.Identification and activation of autocrine renin-angiotensin system in adult ventricular myocytes.Am J Physiol，1995，269(5 pt2)：1791
[3]Wolng A，Clozel JP，Rein J，et al.Functional and biochemical analysis of angiotesin-Ⅱforming pathway：in the human heart.Cir Res，1997，80(2)：219
[4]张琪，丁金凤，赵秀文，等.卡托普利对自发性高血压大鼠心血管组织肾素，血管紧张素的影响.中国循环杂志，1995，10(4)：219, http://www.100md.com
单位：第三军医大学西南医院心内科(400038)
关键词：压力超负荷；肾素-血管紧张素系统；循环；心脏局部组织
重庆医学000410摘 要：目的旨在研究血管紧张转换酶(ACE)抑制剂培哚普利和血管紧张素Ⅱ受体的Ⅰ型受体(AT1R)拮抗剂Irbesartan对循环和心脏局部组织肾素-血管紧张素系统(RAS)影响。方法采用大鼠腹主动脉缩窄模型，用放射免疫法测定血浆肾素(RA)浓度、血管紧张素Ⅱ(AngⅡ)含量，心肌RA浓度、AngⅡ含量；比色法测血清和心肌ACE活性。结果循环AngⅡ含量、ACE活性与LVMⅠ之间均无相关性(r1=0.0967，r2=0.1450，P>0.05)。Irbestarsan组循环中RA、AngⅡ含量和ACE活性显著高于手术组(P<0.01)；培哚普利组循环RA显著高于手术组(P<0.01)，ACE活性和AngⅡ含量显著低于手术组(P<0.01)。手术组心肌AngⅡ含量、ACE活性均与LVMⅠ呈显著正相关(r1=0.8266，r2=0.828P<0.01)。Irbesartan组心肌RA、AngⅡ和ACE均显著低予手术组(P<0.01)；培哚普利组心肌RA显著高于手术组(P<0.05)，但ACE活性和AngⅡ含量显著低予手术组(P<0.01)。结论心脏局部组织RAS而非循环RAS在压力超负荷致心肌肥大中起重要作用，培哚普利和Irbesartan防止心肌肥厚发生的重要机制之一是阻断心脏组织RAS，抑制心肌组织局部AngⅡ的生成。
, http://www.100md.com
Effects of Irbesartan and Peridopril on Circulating and Local Renin-Angiotensin System(RAS) in Rats With Pressure-over-Loading Cardiac Hypertrophy
Zhang Ping ,He Guoxiang ,Chi Luxiang
(Department of Cardiology,Southwest Hospital,Third Military Madical University,Chongqing 400038)
Abstract：Aim:To investigate the effects of Irbesartan and peridopril on circulating and local renin-angiiotension system (RAS)in rats.Methods:Male wistar rats were divided randomly into 4 group:shame-operated(So), abdominal aorta constiction(AC), AC with Irbesartan(AC+ Irb), AC with Peridopril (AC+Per). At 10th week after operation, circulating and ventricular renin activity (RA), angiotensin Ⅱ(Ang Ⅱ), angiotensin I converting enzyme(ACE) activity were measured respectively by radioimmunoassay(RIA) and biochemical analysis. Results:Levels of circulating RA, Ang Ⅱ and ACE in AC+Irb were significantly higher than those in AC (P<0.01), Plasma RA in AC+Per was significantly higher than that in AC(P<0.01 ), plasma Ang Ⅱ and serum ACE were significantly lover than those in AC(0.01). Components of cardiac RAS in AC+Irb were significantly lower than those in AC P<0.05). Compared with AC, ventricular RA in AC+Per was decreased obviously(p<0.01),ACE and Ang Ⅱ were increased obviously(P<0.01).Left ventricular mass index(LVMⅠ) was positivily correlated with both ventricular ACE and Ang Ⅱ(r1=0.8266, r2=0.828 P<0.01), not with circulating ACE and Ang Ⅱ. Conclusion: These results suggest that cardiac RAS plays an important role in the cardiac hypertrophy during pressure over-loading. A machanism of preventing cardiac hypertrophy by Irbesartan and Peridopril is due to blocking cardiac RAS, inhibiting production of ventricular Ang Ⅱ.
, http://www.100md.com
Keywords：Pressure-overload Renin-angiotensin system Circulation Cardiac local tissue
参考文献：
[1]Danser AH.Local renin-angiotensin system.Mol Cell Biochem，1996，167(3)：211
[2]Zhang x，Dostal DE，Reiss K，et al.Identification and activation of autocrine renin-angiotensin system in adult ventricular myocytes.Am J Physiol，1995，269(5 pt2)：1791
[3]Wolng A，Clozel JP，Rein J，et al.Functional and biochemical analysis of angiotesin-Ⅱforming pathway：in the human heart.Cir Res，1997，80(2)：219
[4]张琪，丁金凤，赵秀文，等.卡托普利对自发性高血压大鼠心血管组织肾素，血管紧张素的影响.中国循环杂志，1995，10(4)：219, http://www.100md.com