FITC-Annexin V/PI双染色FCM检测非小细胞肺癌药物敏感性
作者：苏建中 黄强刚 龚乐罗 江晓丰 王恩忠
单位：200030 上海市，上海胸科医院
关键词：化疗药物敏感试验；流式细胞分析法；非小细胞肺癌
中国煤炭工业医学杂志000483摘 要：目的检测非小细胞肺癌(NSCLC)体外化疗药物敏感性。方法采用FITC-AnnexinV/PI双染色双变量流式细胞分析法。结果49例非小细胞肺癌对化疗药物的敏感度由高至低依次为泰素、顺铂、丝裂霉素、诺维本、长春地辛和依托泊苷。结论本试验是一种新的化疗药物敏感性检测方法。6种化疗药物对NSCLC标本的敏感度与临床单剂化疗疗效基本一致，提示该检测对提供临床个体化化疗方案的选择具有重要的参考价值。
Determination of Sensitivity of Non-Small Cell Long Cancer Medicines by FITC--Annexin V/PI double staining Flow Cell Method
Su Jianzhong ,Huang Oianggang ,Gong Leluo
(Shanghai Chest Hospital, Shanghai, 200030)
Abstract：Objective To determine the sensitivity of extrinisic chemical therapy medicines of non small cell lung cancer (NSCLC). Methods FITC-Annexin V/PI double staining double variables flow cell method (FCM) was adopted. Results 49 NSCLC were tested and they were most sensitive to Taxol, and next to it are DDP, MMC, NVB, VDS and VP-16. Conclusion This test is a new way to determine the sensitivity of the chemical therapy medicines. These 6 chemical medicines’ sensitivity to NSCLC samples is almost consistent with the chemical therapy effects of the clinical individual medicine. This determination is of important referring value to decide on the clinical individual chemical therapy alternatives.
Keywords：drug sensitivity test of chemotherapy flow cell method(FCM) non small cell lung cancer (NSCLC)
参考文献：
[1]Easter DW, Halasz NA. Recent trends in the management of desmoid tumors. Ann Surg, 1989,210:765.
[2]I chiro Y,Tokujiro Y, Yasunobu Y, et al. Oligoclonal T lymphocytes infiltrating human lung cancer tissues. Int. J. Cancer, 1991,47:654.
[3]Istvan V, Clemens H, Helga S. N, et al. A novel assay for apoptosis flow cytometrie detection of phosphatidylserine expression on early apoptotie cell using fluorescein labelled Annexin V. J. Immunol Methods, 1995,184:39～51.
[4]廖美琳，主编.肺癌现代治疗.上海：上海医科大学出版社，1998. 278～279.
收稿日期：1999-11-16
修稿日期：1999-12-27, http://www.100md.com
单位：200030 上海市，上海胸科医院
关键词：化疗药物敏感试验；流式细胞分析法；非小细胞肺癌
中国煤炭工业医学杂志000483摘 要：目的检测非小细胞肺癌(NSCLC)体外化疗药物敏感性。方法采用FITC-AnnexinV/PI双染色双变量流式细胞分析法。结果49例非小细胞肺癌对化疗药物的敏感度由高至低依次为泰素、顺铂、丝裂霉素、诺维本、长春地辛和依托泊苷。结论本试验是一种新的化疗药物敏感性检测方法。6种化疗药物对NSCLC标本的敏感度与临床单剂化疗疗效基本一致，提示该检测对提供临床个体化化疗方案的选择具有重要的参考价值。
Determination of Sensitivity of Non-Small Cell Long Cancer Medicines by FITC--Annexin V/PI double staining Flow Cell Method
Su Jianzhong ,Huang Oianggang ,Gong Leluo
(Shanghai Chest Hospital, Shanghai, 200030)
Abstract：Objective To determine the sensitivity of extrinisic chemical therapy medicines of non small cell lung cancer (NSCLC). Methods FITC-Annexin V/PI double staining double variables flow cell method (FCM) was adopted. Results 49 NSCLC were tested and they were most sensitive to Taxol, and next to it are DDP, MMC, NVB, VDS and VP-16. Conclusion This test is a new way to determine the sensitivity of the chemical therapy medicines. These 6 chemical medicines’ sensitivity to NSCLC samples is almost consistent with the chemical therapy effects of the clinical individual medicine. This determination is of important referring value to decide on the clinical individual chemical therapy alternatives.
Keywords：drug sensitivity test of chemotherapy flow cell method(FCM) non small cell lung cancer (NSCLC)
参考文献：
[1]Easter DW, Halasz NA. Recent trends in the management of desmoid tumors. Ann Surg, 1989,210:765.
[2]I chiro Y,Tokujiro Y, Yasunobu Y, et al. Oligoclonal T lymphocytes infiltrating human lung cancer tissues. Int. J. Cancer, 1991,47:654.
[3]Istvan V, Clemens H, Helga S. N, et al. A novel assay for apoptosis flow cytometrie detection of phosphatidylserine expression on early apoptotie cell using fluorescein labelled Annexin V. J. Immunol Methods, 1995,184:39～51.
[4]廖美琳，主编.肺癌现代治疗.上海：上海医科大学出版社，1998. 278～279.
收稿日期：1999-11-16
修稿日期：1999-12-27, http://www.100md.com