深低温冷冻异体骨生物力学特性与储存时间的关系
作者：杨克强 王臻 桑宏勋 吕昌伟 王海强 彭磊
单位：杨克强(解放军第255医院外二科 唐山 063000)；王臻 桑宏勋 吕昌伟 王海强 彭磊(第四军医大学西京医院全军骨科研究所 710032)
关键词：深低温冷冻骨；生物力学；同种异体骨移植
医用生物力学000312摘 要：目的：研究深低温冷冻法储骨时间与皮质骨生物力学特性的关系。方法：用深低温冷冻法储存的腓骨皮质作标本，分别将储存1年和7年的腓骨皮质做成弯曲及压缩试件，在Instron1195型电子拉伸试验机上测试两组的极限弯曲强度，弹性模量及极限压缩强度，所得数据进行组间t检验。结果：1年组和7年纽的极限弯曲强度差异不明显(P>0.05)，弹性模量差异不明显(P>0.05)，极限压缩强度差异不明显(P>0.05)。结论：深低温冷冻法储骨，皮质骨的生物力学特性受储存时间长短影响不显著。只要确保无污染，储存骨的使用不应受储存时间长短的影响，术中术后处理亦不应受影响。 THE RELATIONSHIP BETWEEN BIOMECHANICAL PROPERTIES OF DEEP FROZEN CRYOPRESERVATING ALLOGRAFT AND THE PRESERVATING TIME
, http://www.100md.com
Yang Ke-Qiang ,Wang Zhen ,Sang Hong-Xun
(Department of Orthopedics, 255th Hospital of PLA, TANG SHAN, HEBEI, 063000)
Abstract：Objective: To study the relationship between the preservating time of deep frozen cryopreservating allograft and the biomechanical properties. Method: Specimens of the fibular bones of deep frozen cryopreservation for 1 and 7 years were made into test pieces of torture and compaction. And the bending strength(BS), modulus of elasticity(ME) and compact strength(CS) were measured on the INSTRON1195 electronic tensile test machine. The results were analysised with T test. Results: The 1 year group and the 7 year group had no significant difference in terms of BS (P>0.05), ME(P>0.05) and CS(P>0.05). Conclusion: Cryopreservation had no offects on the cortical bone biomechanics, as long as not being contaminated, the cryopreservation will be independent of storage time and the pre-or pro-operation treatments.
, 百拇医药
Keywords：Deep Frozen Bone, Biomechanics, Allografts
作者简介：杨克强 第四军医大学骨科研究所部队临床医学中青年人才培养基金班学员
参考文献：
[1]Finn HA. Technical Aspects and surgical complication of segmental allografts Following segmental resection of maligmant bone tumors. In: Langlais F. Tomenoed B. Limb salvage-major reconstructions in oncologic and nontumoral conditions.Verlag Berlin:Springer Heidelberg, 1991, 75～87.
, 百拇医药
[2]Mizutani H, and Urist MR, The nature of bone morphogenetic protein(BMP)fractions derived from bovine bone matrix gelatin, Clin Orthop, 1982; 171: 213.
[3]任祖翰，骨与软骨的低温保存.见：刘金刚，低温医学.北京，人民卫生出版社.1993: 213.
[4]郭仁棣，张宏民，杨育勇.国人尺骨静态弹性范围内力学性的测定.中国生物医学工程学报.1994; 13(1): 74-77.
[5]Komender A. Infiuence of preservation on soms mechanical propertis of human haversian bone. Mater Med Pol 1976; 8: 13.
, 百拇医药
[6]Pelker RR. Friedlaender GD. Markham TC. Biomechanical properties of bone allografts. Clin Orthop 1983; 174: 54.
[7]Ma ZG, Zhou SX, Liu BL.The mechanical and biological properties of deminera-liged cortical bone allografts in animals. J Bone Joint Surg (Br), 1991, 73: 791.
[8]Urist MR. Bone formation in implants of partially and wholly demincralized bone matrix. Clin Othop, 1970, 71: 27.
[9]Tomford WW, Doppelt SH, Mankin HJ, et al. Bone bank procedures. Clin Orthop. 1983; 174: 15., 百拇医药
单位：杨克强(解放军第255医院外二科 唐山 063000)；王臻 桑宏勋 吕昌伟 王海强 彭磊(第四军医大学西京医院全军骨科研究所 710032)
关键词：深低温冷冻骨；生物力学；同种异体骨移植
医用生物力学000312摘 要：目的：研究深低温冷冻法储骨时间与皮质骨生物力学特性的关系。方法：用深低温冷冻法储存的腓骨皮质作标本，分别将储存1年和7年的腓骨皮质做成弯曲及压缩试件，在Instron1195型电子拉伸试验机上测试两组的极限弯曲强度，弹性模量及极限压缩强度，所得数据进行组间t检验。结果：1年组和7年纽的极限弯曲强度差异不明显(P>0.05)，弹性模量差异不明显(P>0.05)，极限压缩强度差异不明显(P>0.05)。结论：深低温冷冻法储骨，皮质骨的生物力学特性受储存时间长短影响不显著。只要确保无污染，储存骨的使用不应受储存时间长短的影响，术中术后处理亦不应受影响。 THE RELATIONSHIP BETWEEN BIOMECHANICAL PROPERTIES OF DEEP FROZEN CRYOPRESERVATING ALLOGRAFT AND THE PRESERVATING TIME
, http://www.100md.com
Yang Ke-Qiang ,Wang Zhen ,Sang Hong-Xun
(Department of Orthopedics, 255th Hospital of PLA, TANG SHAN, HEBEI, 063000)
Abstract：Objective: To study the relationship between the preservating time of deep frozen cryopreservating allograft and the biomechanical properties. Method: Specimens of the fibular bones of deep frozen cryopreservation for 1 and 7 years were made into test pieces of torture and compaction. And the bending strength(BS), modulus of elasticity(ME) and compact strength(CS) were measured on the INSTRON1195 electronic tensile test machine. The results were analysised with T test. Results: The 1 year group and the 7 year group had no significant difference in terms of BS (P>0.05), ME(P>0.05) and CS(P>0.05). Conclusion: Cryopreservation had no offects on the cortical bone biomechanics, as long as not being contaminated, the cryopreservation will be independent of storage time and the pre-or pro-operation treatments.
, 百拇医药
Keywords：Deep Frozen Bone, Biomechanics, Allografts
作者简介：杨克强 第四军医大学骨科研究所部队临床医学中青年人才培养基金班学员
参考文献：
[1]Finn HA. Technical Aspects and surgical complication of segmental allografts Following segmental resection of maligmant bone tumors. In: Langlais F. Tomenoed B. Limb salvage-major reconstructions in oncologic and nontumoral conditions.Verlag Berlin:Springer Heidelberg, 1991, 75～87.
, 百拇医药
[2]Mizutani H, and Urist MR, The nature of bone morphogenetic protein(BMP)fractions derived from bovine bone matrix gelatin, Clin Orthop, 1982; 171: 213.
[3]任祖翰，骨与软骨的低温保存.见：刘金刚，低温医学.北京，人民卫生出版社.1993: 213.
[4]郭仁棣，张宏民，杨育勇.国人尺骨静态弹性范围内力学性的测定.中国生物医学工程学报.1994; 13(1): 74-77.
[5]Komender A. Infiuence of preservation on soms mechanical propertis of human haversian bone. Mater Med Pol 1976; 8: 13.
, 百拇医药
[6]Pelker RR. Friedlaender GD. Markham TC. Biomechanical properties of bone allografts. Clin Orthop 1983; 174: 54.
[7]Ma ZG, Zhou SX, Liu BL.The mechanical and biological properties of deminera-liged cortical bone allografts in animals. J Bone Joint Surg (Br), 1991, 73: 791.
[8]Urist MR. Bone formation in implants of partially and wholly demincralized bone matrix. Clin Othop, 1970, 71: 27.
[9]Tomford WW, Doppelt SH, Mankin HJ, et al. Bone bank procedures. Clin Orthop. 1983; 174: 15., 百拇医药