Ala-Gln和BCAA对创伤大鼠静脉营养支持作用的实验研究
作者：靳继德 徐琪寿 韦京豫 郭长江 郑子新
单位：靳继德 徐琪寿 韦京豫 郭长江(军事医学科学院放射医学研究所)；郑子新(解放军301医院营养科)
关键词：
中国临床营养杂志000110摘 要：目的观察富含丙氨酰谷氨酰胺(Ala-Gln)和支链氨基酸(BCAA)的氨基酸注射液对创伤大鼠的静脉营养支持效果。 方法Wistar大鼠40只，行创伤和中心静脉插管后，随机分为无氮组、正常饲料组、对照组和实验组。测定饲料摄入量、体重、氮平衡、血浆蛋白和氨基酸含量、外周血淋巴细胞转化率、伤口埋植海绵中羟脯氨酸含量、皮肤抗张力强度和小肠粘膜蛋白质及核酸。 结果无氮组动物营养状况欠佳，各项指标均较差。实验组动物的血浆谷氨酰胺(Gln)、BCAA含量，外周血淋巴细胞转化率，伤口皮肤抗张力强度和羟脯氨酸含量都显著高于对照组和正常饲料组(P<0.05)；实验组和正常饲料组动物第8天氮平衡，小肠粘膜核酸和蛋白质明显高于对照组(P<0.05)。 结论Gln和BCAA有助于改善创伤后机体的营养及代谢状况，增强免疫功能，促进小肠粘膜细胞增殖。
Effect of Ala-Gln and BCAA on traumatized rat
JIN Jide ,XU Qishou ,ZHENG Zixin
(Institute of Radiation Medicine, Academy of Military Medical Science, Beijing , 100850)
Abstract：Purpose To observe the effects of amino acids solution supplimenteel with alanylglutamine (Ala-Gln) and branched-chain amino acids (BCAA) on traumatized rats. Methods After the injuny, and centeral vena catheter placed, 40 male, Wistar rats were randomly divided into nitrogen-free, normal diet, control and experimental groups. Dietary intake was weighed everyday, and body weight was checked every other day. On days 4 and 8, nitrogen balance was measured. On days 14, serum proteins and amino acids concentration and peripheral lymphocyte blastogenesis were determined. At the end of the study, the animals were sacrificed, hydroxyproline and tensile strength of wound, and intestinal mucosal DNA, RNA and protein content were examined. Results Nitrogenfree group was worse in all variance than other groups. The nitrogen balance on days 8 was remarkably improved in experimental and normal diet groups compared with control group. There were highter serum glutamine (Gln) and BCAA concentration, hydroxyproline content and tensile strength of wound, and better lymphocyte blastogenesis in experimental group compared with control and normal diet groups. Experimental and normal diet groups had higher mucosal DNA, RNA and protein content than control group. Conclusions Gin and BCAA can optimize hypercatabolism, enhance immunity, and promote wound healing and the growth of intestinal mucosa., 百拇医药
单位：靳继德 徐琪寿 韦京豫 郭长江(军事医学科学院放射医学研究所)；郑子新(解放军301医院营养科)
关键词：
中国临床营养杂志000110摘 要：目的观察富含丙氨酰谷氨酰胺(Ala-Gln)和支链氨基酸(BCAA)的氨基酸注射液对创伤大鼠的静脉营养支持效果。 方法Wistar大鼠40只，行创伤和中心静脉插管后，随机分为无氮组、正常饲料组、对照组和实验组。测定饲料摄入量、体重、氮平衡、血浆蛋白和氨基酸含量、外周血淋巴细胞转化率、伤口埋植海绵中羟脯氨酸含量、皮肤抗张力强度和小肠粘膜蛋白质及核酸。 结果无氮组动物营养状况欠佳，各项指标均较差。实验组动物的血浆谷氨酰胺(Gln)、BCAA含量，外周血淋巴细胞转化率，伤口皮肤抗张力强度和羟脯氨酸含量都显著高于对照组和正常饲料组(P<0.05)；实验组和正常饲料组动物第8天氮平衡，小肠粘膜核酸和蛋白质明显高于对照组(P<0.05)。 结论Gln和BCAA有助于改善创伤后机体的营养及代谢状况，增强免疫功能，促进小肠粘膜细胞增殖。
Effect of Ala-Gln and BCAA on traumatized rat
JIN Jide ,XU Qishou ,ZHENG Zixin
(Institute of Radiation Medicine, Academy of Military Medical Science, Beijing , 100850)
Abstract：Purpose To observe the effects of amino acids solution supplimenteel with alanylglutamine (Ala-Gln) and branched-chain amino acids (BCAA) on traumatized rats. Methods After the injuny, and centeral vena catheter placed, 40 male, Wistar rats were randomly divided into nitrogen-free, normal diet, control and experimental groups. Dietary intake was weighed everyday, and body weight was checked every other day. On days 4 and 8, nitrogen balance was measured. On days 14, serum proteins and amino acids concentration and peripheral lymphocyte blastogenesis were determined. At the end of the study, the animals were sacrificed, hydroxyproline and tensile strength of wound, and intestinal mucosal DNA, RNA and protein content were examined. Results Nitrogenfree group was worse in all variance than other groups. The nitrogen balance on days 8 was remarkably improved in experimental and normal diet groups compared with control group. There were highter serum glutamine (Gln) and BCAA concentration, hydroxyproline content and tensile strength of wound, and better lymphocyte blastogenesis in experimental group compared with control and normal diet groups. Experimental and normal diet groups had higher mucosal DNA, RNA and protein content than control group. Conclusions Gin and BCAA can optimize hypercatabolism, enhance immunity, and promote wound healing and the growth of intestinal mucosa., 百拇医药